Amplicon-based target enrichment methods for next-generation sequencing (NGS) rely on PCR-amplification of target regions of interest using sequence-specific primers and probes. Using molecular inversion probes (MIP), specific probes consisting of a universal linker flanked by target-specific sequences are constructed. The probes anneal on both sides of the target amplicon and the gap is filled by a DNA polymerase.
Roche Sequencing Solutions offers the HEAT-Seq® portfolio of target enrichment products, which utilize an advanced version of MIP technology, for single nucleotide variation (SNV) detection for targeted sequencing applications in human genetic disease and cancer research.