CONFIRM anti-Topoisomerase IIα (JS5B4) Rabbit Monoclonal Primary Antibody

This antibody is intended for in vitro diagnostic (IVD) use.

Ventana Medical Systems' CONFIRM anti-Topoisomerase IIα (JS5B4) Rabbit Monoclonal Primary Antibody is designed to qualitatively detect the presence of topoisomerase IIα expressing cells via light microscopy in formalin-fixed, paraffin-embedded tissue following staining on a Ventana BenchMark XT instrument. During the cell cycle, topoisomerase IIα is expressed in the G1, S, G2, and M phase but is absent in the G0 (quiescent) phase.1 Assessment of the proportion of positive staining cells for topoisomerase IIα may aid in evaluation of the proliferative activity of normal and/or neoplastic tissue.2,3,4 The clinical interpretation of any staining, or the absence of staining, must be complemented by morphological studies and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.

1. Kreipe H, Heidebrecht HJ, Hansen S, Röhlk W, Kubbres M, Wacker HH, et al. A new proliferation associated nuclear antigen detectable in paraffin embedded tissues by monoclonal antibody Ki-S1. AM J. Pathol 1993; 142:3-9.

2. Rudolph P, Lappe T, Scubert C, Schmidt D, Parwaresch RM, Christophers E. Diagnostic assessment of two novel proliferation –specific antigens in benign and malignant melanocytic lesions. AM J. Pathol 1995; 147:1615-25.

3. Kreipe H, Alm P, Olsson H, Hauberg M, Fisher L, Parwaresch RM. Prognostic significance of a formalin resistance nuclear proliferation antigen in mammary carcinoma as determined by the monoclonal antibody Ki-S1. AM J Pathol 1993; 142:651-657.

4. Kellner U, Heidebrecht HJ, Rudolph P, et al. Detection of human Topoisomerase IIα in cell lines and tissue: Characterization of five novel monoclonal antibodies. J of Histochem and Cytochem 1997; 45:251-264.

5. Barshi RP, Galande S, Muneyappa K. Functional and regulatory characteristics of eukaryotic type II DNA Topoisomerase. Critical Rev Biochem Mol Biol 2001; 36:1- 37.

6. Woessner RD, Mattern MR, Merbelli CK, Johnson RK, Drake FH. Proliferation and cell cycle dependent differences in expression of the 170kD and 180kD forms of Topoisomerase II in NIH3T3 cells. Cell Growth Differ 1991; 2:209-214. S

7. heehan DC, Hrapchak BB. Theory and Practice of Histotechnology, 2nd Edition. The C.V. Mosby Company, St. Louis, 1980.

8. Roche PC, Hsi ED. Immunohistochemistry-Principles and Advances. Manual of Clinical Laboratory Immunology, 6th edition. (NR Rose Ed.) ASM Press, 2002.