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2-8°C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Background Information", "Value": "p27^Kip1, also known as cyclin-dependent kinase inhibitor 1B (CDNK1B), is a kinase inhibitor that controls cell cycle progression.^1-4 p27^Kip1 is involved in G1 phase arrest and obstructs cell entry into the S phase by binding to and inhibiting cyclin E-CDK2, effectively slowing or stopping the cell division cycle.^1-4 p27^Kip1 is broadly expressed in normal tissue but can be dysfunctional in neoplastic tissue and, therefore, not expressed.^1-2

1. Polyak K, et al. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell. 1994; 78:59-66.
2. Sun C, et al. Regulation of p27Kip1 phosphorylation and G1 cell cycle progression by protein phosphatase PPM1G. AM J Cancer Res. 2016; 6:2207-20.
3. Sangfelt O, et al. Molecular mechanisms underlying interferon-alpha-induced G0/G1 arrest: CKI-mediated regulation of G1 Cdk-complexes and activation of pocket proteins. Oncogene. 1999; 18:2798-810.
4. Hsieh FF, et al. Cell cycle exit during terminal erythroid differentiation is associated with accumulation of p27(Kip1) and inactivation of cdk2 kinase. Blood. 2000; 96:2746-54.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Intended Use", "Value": "L’anticorps primaire monoclonal de souris de la p27^Kip1 (SX53G8) est destiné à l’usage en laboratoire pour la détection de la protéine p27^Kip1 dans du tissu fixé au formaldéhyde, inclus dans la paraffine, coloré sur les instruments (IHC/ISH) VENTANA BenchMark. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "p27^Kip1 (SX53G8) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "L’anticorps primaire monoclonal de souris de la p27^Kip1 (SX53G8) est destiné à l’usage en laboratoire pour la détection de la protéine p27^Kip1 dans du tissu fixé au formaldéhyde, inclus dans la paraffine, coloré sur les instruments (IHC/ISH) VENTANA BenchMark. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Product Purpose" } ] } } ] }