Lung cancer IHC portfolio

Lung assays
Deliver diagnostic confidence with the lung IHC portfolio

Roche Diagnostics offers a comprehensive menu of lung cancer diagnostic solutions. Our portfolio of IHC diagnostic assays, IHC staining products, and more, deliver the high sensitivity and specificity you need.

VENTANA prediluted, optimised reagents

Our antibodies are ready-to-use on the fully automated VENTANA BenchMark IHC/ISH staining platforms, reducing the time-to-result and resources required with manual or semi-automated solutions. VENTANA prediluted, optimised reagents consistently demonstrate better performance when compared with concentrated antibodies that require manual dilution and in-house validation.

Our portfolio of lung cancer assays can assist in the stratification of disease, including non-small cell lung carcinoma (NSCLC), neuroendocrine carcinoma and their various subtypes.

VENTANA ROS1 (SP384) Rabbit Monoclonal Primary Antibody
  • The first and only pre-optimised, ready-to-use IVD IHC ROS1 assay on the market
  • Highly correlated to FISH break-apart status (92.5% OPA)1
  • High correlation between pathologists (96.4% OPA)2
p40 (BC28) Mouse Monoclonal Primary Antibody


  • Found to be as or more sensitive than p634,5,6
  • Studies indicate p40 has higher specificity in squamous cell carcinoma than p634,5,6
  • May offer a suitable replacement for p63 in various tumour types7,8
  • Can be used in a stratification panel with other key markers9,10
immunohistochemical staining with Basal Cell Cocktail 34ßE12 + p63
TTF-1 (SP141) Rabbit Monoclonal Primary Antibody


  • Aids in investigation of metastatic carcinomas of unknown origin11
  • Equal or better lung carcinoid detection than other on-market clones12
  • Commonly used in lung stratification panels with other key markers9,10
immunohistochemical staining with Basal Cell Cocktail 34ßE12 + p63
Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody


  • Aids in the identification of squamous cell carcinoma13
  • Aids in the differentiation of malignant mesothelioma from lung adenocarcinoma14
  • Commonly used in a stratification panel with other markers9,10
immunohistochemical staining with Basal Cell Cocktail 34ßE12 + p63
Napsin A (MRQ-60) Mouse Monoclonal Primary Antibody


  • Useful in the identification of primary and metastatic carcinomas of the lung15
  • Highly specific for lung adenocarcinoma9
  • Commonly used in a lung stratification panel with other key markers9,10



  1. Huang, R. et al. P2.09-13 Correlation of ROS1 (SP384) Immunohistochemistry with ROS1 Rearrangement Determined by Fluorescence In Situ Hybridization. IASLC 2018 19th World Conference on Lung Cancer Abstracts. Accessed 6th March, 2019. 
  2. Hanlon Newell, A. et al. MA26.07 ROS1 (SP384) Immunohistochemistry Inter-Reader Precision Between 12 Pathologists. IASLC 2018 19th World Conference on Lung Cancer Abstracts. Accessed 6th March, 2019.
  3. Assessment Run 48 p40 (ΔNp63) NordiQC. Updated 2016, accessed 21 MAR 2017
  4. Bishop, Justin A, Julie Teruya-Feldstein, William H Westra, Giuseppe Pelosi, William D Travis and Natasha Rekhtman. p40 (∆Np63) is superior to p63 for the diagnosis of pulmonary squamous cell carcinoma. Modern Pathology. 2012 Mar;25(3):405-15.
  5. Chen, Xiangbai, Yunhau Bao, Rebecca Wong, Katerina Politi, Lizhuo Jiao, Scott Gettinger, Robert Homer, David Chieng, Guoping Cai. [351] The Role of p40 Immunostain in the Cytological Differential Diagnosis of Squamous Cell Carcinoma and Adenocarcinoma of the Lung. USCAP Abstract. 2013
  6. Vogt, Adam P, Cynthia Cohen, Momin T Siddiqui. [436] p40 (ΔNp63): A Highly Sensitive and Specific Immunohistochemical (IHC) Marker for Diagnosing Pulmonary Squamous Cell Carcinomas (SQCC) in Fine Needle Aspirates. USCAP Abstract. 2013.
  7. Tacha, David PhD; Ryan Bremer, PhD; Thomas Haas, DO; Weiman Qi, PhD, MD. An Immunohistochemical Analysis of a Newly Developed, Mouse Monoclonal p40 (BC28) Antibody in Lung, Bladder, Skin, Breast, Prostate and Head and Neck Cancers. Arch Pathol Lab Med—Vol 138, October 2014.
  8. Sailer et al. Comparison of p40 (DNp63) and p63 expression in prostate tissues – which one is the superior diagnostic marker for basal cells? Histopathology. 2013. 63: 50–56.
  9. Wei Zhao, Hui Wang, Yan Peng, Bo Tian, Lei Peng, Da-Chuan Zhang. ΔNp63, CK5/6, TTF-1 and napsin A, a reliable panel to subtype non-small cell lung cancer in biopsy specimens. Int J Clin Exp Pathol. 2014; 7(7): 4247–4253.
  10. Kai Zhang, Hongbin Deng, and Philip T. Cagle (2014) Utility of Immunohistochemistry in the Diagnosis of Pleuropulmonary and Mediastinal Cancers: A Review and Update. Archives of Pathology & Laboratory Medicine: December 2014, Vol. 138, No. 12, pp. 1611-1628.
  11. Daniel Misch, Torsten Blum, Christian Boch, Timo Weiss, Catharina Crolow, Sergej Griff, Thomas Mairinger, Torsten T Bauer and Jens Kollmeier. Value of thyroid transcription factor (TTF)-1 for diagnosis and prognosis of patients with locally advanced or metastatic small cell lung cancer. Diagnostic Pathology (2015) 10:21
  12. Lung cancer diagnostic solutions product brochure (7009A-5)
  13. A. Kargi, D. Gurel, B. Tuna, The diagnostic value of TTF-1, CK 5/6, and p63 immunostaining in classification of lung carcinomas. Appl Immunohistochem Mol Morphol. 2007 Dec;15(4):415-20.
  14. N.G. Ordòñez. Value of cytokeratin 5/6 immunostaining in distinguishing epithelial mesothelioma of the pleura from lung adenocarcinoma. Am J Surg Pathol. 1998 Oct;22(10):1215-21.
  15. Ye J, Findeis-Hosey JJ, Yang Q, McMahon LA, Yao JL, Li F, Xu H. Combination of napsin A and TTF-1 immunohistochemistry helps in differentiating primary lung adenocarcinoma from metastatic carcinoma in the lung. Appl Immunohistochem Mol Morphol. 2011 Jul;19(4):313-7.
  16. Assessment run 45 ALK. NordiQC. Updated 2015, accessed 21 MAR 2017