Elecsys® Phospho-Tau (181P) CSF

ElectroChemiLuminescence Immunoassay (ECLIA) for the in vitro quantitative determination of phosphorylated Tau in human Cerebrospinal Fluid (CSF)

Elecsys® Phospho-Tau (181P) CSF

ElectroChemiLuminescence Immunoassay (ECLIA) for the in vitro quantitative determination of phosphorylated Tau in human Cerebrospinal Fluid (CSF)

Tau (tubulin-associated unit) protein is one of the two hallmarks of Alzheimer’s disease, besides β-Amyloid (1-42) (Abeta42). During neurodegeneration abnormal phosphorylation leads to formation of intracellular neurofibrillary tangles (NFTs) composed of the Tau protein that has undergone hyper-phosphorylation, and developed aggregates of hyper-phosphorylated Tau proteins called Phospho-Tau (pTau)1,2

The Elecsys Phospho-Tau (181P) CSF assay is designed to detect the protein or fragments of Tau protein phosphorylated at threonine 181 in human CSF.

Many studies show that high CSF pTau levels is associated with a faster progression from Mild Cognitive Impairment (MCI) to AD with more rapid cognitive decline in AD patients3 and in mild AD dementia cases.4 CSF pTau levels increase around 2 – 3 fold in mild‑moderate AD patients compared to age‑matched controls, while CSF β‑Amyloid (1‑42) (Abeta42) levels decrease to around half the level in controls.5,6

 

Platform availability

MODULAR ANALYTICS E170, cobas e 601, cobas e 602, cobas e 411

Elecsys® Phospho-Tau (181P) CSF

Elecsys® Phospho-Tau (181P) CSF

  • Systems

    cobas e 411 analyzer, cobas e 601 / cobas e 602 modules, MODULAR ANALYTICS E170

  • Testing Time

    18 minutes

  • Test principle

    Sandwich principle

  • Calibration

    2-point

  • Traceability

    LC-MS/MSTau (172-205) [pThr181]amid

  • Sample material

    Human Cerebrospinal Fluid (CSF)

  • Sample volume

    50 μL

  • LoB (Limit of Blank)

    4 pg/mL

  • LoD (Limit of Detection)

    8 pg/mL

  • LoQ (Limit of Quantitation)

    8 pg/mL

  • Measuring range

    8-120 pg/mL (defined by the Limit of Quantitation and the maximum of the master curve)

    Values below the Limit of Quantitation are reported as < 8 pg/mL. 

    Values above the measuring range are reported as > 120 pg/mL

  • Intermediate precision

    cobas e 411: 2.1-6.7% (0.278-3.87 pg/mL)

    E170, cobas e 601, cobas e 602: 1.9% -3.5% (0.313 – 3.45 pg/mL)

References

 

  1. Iqbal, K., Liu, F., Gong, CX. (2016). Nat Rev Neurol, 12(1), 15-27. 
  2. Wang, Y., Mandelkow, E. (2016). Nat Rev Neurosci, 17(1), 5-21.
  3. Blom, ES., Giedraitis, V., Zetterberg, H. et al. (2009). Dement Geriatr Cogn Disord, 27(5), 458-64.
  4. Snider, BJ., Fagan, AM., Roe, C. et al. (2009). Arch Neurol, 66(5), 638-45.
  5. Mattsson, N., Zetterberg, H., Hansson, O., et.al. (2009). JAMA 22, 302(4), 385-93. 
  6. Hampel, H., Blennow, K. (2004). Dialogues Clin Neurosci,6(4), 379-390.