MagNA Pure FFPET Buffer Set

MagNA Pure FFPET

The MagNA Pure FFPET Buffer Set is a FFPET pretreatment reagent set to enable nucleic acid purification in automated and semi-automated workflows. When used with MagNA Pure 24 System, it gives an exclusive feature for a fully automated FFPET sample extraction workflow.  

Benefits

 

  • Elimination of hazardous and odor for deparaffinization
  • Deparaffinization and lysis of formalin-fixed paraffin-embedded tissue samples in automated and manual nucleic acid isolation workflows
  • A pretreatment solution compatible with FFPET protocols on MagNA Pure Systems
  • Simplicity with only three components for easy setup
  • Flexibility across a broader range of genomic applications such as real-time PCR and sequencing

Registration status
 

For general laboratory use.

Package inserts
 

Access package inserts through your country’s Roche Diagnostics website.

Download the instructional for use here

For more information about MagNA Pure Systems and products, click below
MagNA Pure Systems

Specifications

 

The MagNA Pure FFPET Buffer Set contains all components needed for FFPET deparaffinization and lysis with no need to order extra products. The Buffer Set is used upstream of extraction, similar to other sample pre-treatment buffers.

  • Deparaffinization reagent, one bottle
  • Lysis Buffer, two bottles
  • Isopropanol, four bottles
  • 1 kit contains 2 sets  
  • 1 kit processes up to 48 samples in automated workflows or up to 200 samples in manual pretreatment workflow
  • Product no. 08447144001

Application

The MagNA Pure FFPET Buffer Set is a set of reagents intended for general laboratory use to enable isolation of DNA and RNA from FFPET samples. The buffer is compatible with manual or automated nucleic acid extraction, such as the MagNA Pure 96 and the MagNA Pure 24 Systems. FFPET DNA/RNA are the samples of interest in downstream applications in oncology studies.

Details

When used with the MagNA Pure 24 System, the MagNA Pure FFPET Buffer Set is used on-board as pretreatment reagents to support nucleic acid purification with the MagNA Pure 24 Total NA Isolation Kit (07 658 036 001) and MagNA Pure 24 DNA FFPET 1000 protocol. 

When used with the MagNA Pure 96 System, the Buffer Set is used for off-board pretreatment of FFPET samples. In the following workflow on MagNA Pure 96 System, MagNA Pure 96 DNA and Viral NA Small Volume Kit (06 543 588 001) for the DNA FFPE SV protocol or MagNA Pure 96 Cellular RNA Large Volume Kit (05 467 535 001) for RNA Tissue FFPE LV protocol.

MagNA Pure 24 FFPET workflow.

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MagNA Pure 24 FFPET workflow.

FFPET protocol on MagNA Pure 24 System FFPET sample input (µm), elution volume (µL)
DNA FFPET 1000
  • Start: up to 6 mm3   formalin-fixed   paraffin-embedded     tissue, corresponding   to 6   FFPET sections of   4 or 5 µm
  • Elution: 50 µL or 100 µL

MagNA Pure 96 FFPET workflow.

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MagNA Pure 96 FFPET workflow.

 

FFPET protocols on MagNA Pure 96 System
FFPET sample input (µm), elution volume (µL)
DNA FFPET SV   
  • Start: 2 sections of 5 µm formalin-fixed paraffin-embedded tissue
  • Elution: 50 µL or 100 µL
 RNA Tissue FFPE LV
  • Start: 2 sections of 5 µm formalin-fixed paraffin-embedded tissue
  • Elution: 50 µL or 100 µL