AXELIOS 1 library prep solutions

Prepare high-quality libraries for efficient next-generation sequencing on AXELIOS 1 Platform.

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Library preparation kits for high-performance NGS on the AXELIOS 1 Platform

Solution

Optimal performance and flexibility

AXELIOS 1 research use only library preparation kits provide a robust, comprehensive solution for diverse sample inputs and a broad range of sequencing workflows. It begins with flexible fragmentation using AXELIOS 1 Core Library Prep Kit, followed by dual ligation of AXELIOS 1 Hairpin Adapters (containing sample indices) and the AXELIOS 1 Universal Y-adapter.

By employing linear amplification instead of traditional PCR, this workflow minimizes amplification bias while maintaining the physical link between original duplex strands to achieve higher accuracy.1,2 The process concludes with accurate, Qubit®-compatible quantification, ensuring that high-quality libraries are ready for loading onto the synthesis instrument.3

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  • AXELIOS 1 Core Library Prep Kit: Includes core library preparation reagents for both DNA and cDNA (from RNA), as well as mechanical and enzymatic fragmentation inputs. This kit supports a wide range of input concentration and sample types, and can be used in conjunction with accessory reagents for specific workflows to make libraries that are ready for Xpandomer synthesis.
  • AXELIOS 1 Hairpin Adapters: Includes hairpin adapters that contain sample indices and enable the physical linkage of the original parent-parent strands, creating a duplex library molecule. This supports high consensus accuracy sequencing.3
  • AXELIOS 1 Universal Y-Adapter: Includes Universal Y-adapter containing sequence motifs for synthesis chip binding for Xpandomer synthesis.
  • AXELIOS 1 Library Amplification Kit: Includes reagents for lower bias amplification of libraries preceding Xpandomer synthesis.1,2
  • AXELIOS 1 Library Quantification Kit: Includes a quantification solution and two quantification standards. This molecular beacon-based quantification module is compatible with use on Qubit® 3.0 and 4.0. This module selectively targets the sequence motif required for synthesis chip binding (on the various AXELIOS 1 Y-Adapters), ensuring the precise quantification of only those molecules viable for Xpandomer synthesis and subsequent sequencing on the AXELIOS 1 Platform.

 

Value

Elevate your sequencing performance with flexible and robust AXELIOS 1 solutions

Designed to minimize errors and maximize user flexibility, the AXELIOS 1 library prep solution allows you to go from sample to high-quality sequencing data with confidence, across diverse research applications. 

With our comprehensive solutions, you can:

  • Expand your application range: Balance throughput and application needs using universal reagent kits that support both simplex and duplex sequencing modes. To find out more about previously demonstrated methods, visit our SBX technology webpage.
  • Optimize your operational flexibility: Optimize resource usage and reduce waste by procuring reagents separately, allowing you to align inventory with your workflow and your lab's specific needs.
  • Maximize your efficiency: Ensure the highest yields from even challenging, low-input samples by leveraging industry-leading KAPA enzymes.3
  • Integrate easily: Automation-friendly reagents for multiple on-market liquid handlers.3
Vision

Empowering the next great genomic breakthrough

Your samples hold the potential for the next major breakthrough; our mission is to help you unlock it. By relentlessly pushing the boundaries of NGS library preparation, we deliver the high-performance tools necessary to ensure your sequencing is more accurate and efficient than ever before.3 We are your dedicated partner in science.

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Benefits of AXELIOS 1 library prep solutions from Roche

High-performing

Confident genomic insights. Built on uncompromising performance.

Analytical excellence is at the core of every Roche sequencing solution—engineered to evolve alongside your research.

 

  • Designed for precision: Achieve high-quality accuracy for your critical research demands with the AXELIOS 1 Platform* and its groundbreaking SBX technology.3 
  • The ultimate ecosystem fit: Maximize your platform's potential with AXELIOS 1 library prep reagents, uniquely formulated to drive optimal efficiency and superior results within the AXELIOS 1 ecosystem.

 

Efficient

Don’t compromise between depth and speed

Next-generation sequencing (NGS) delivers unmatched biological insights, but historically at the cost of turnaround time. Roche bridges this gap by continuously optimizing our workflows, chemistries, and analysis algorithms—drastically accelerating your time to result without compromising the data integrity and high confidence your research demands. 

  • SBX technology redefines sequencing efficiency by supporting multiple methods and flexible read lengths on a single platform, all while reducing operational costs via reusable sensor modules. 
  • Our SBX-D library preparation optimizes sequencing economy right from the start by utilizing innovative hairpin adapter ligation to enable true parent-parent duplex tracking.3 This unique prep chemistry incorporates a linear amplification step, ensuring that library errors are not carried through and exponentially multiplied as with traditional PCR.1,2 The result is a library workflow that minimizes error carry-over and delivers reliable data with maximum accuracy.
Automation-friendly

Boost your throughput—without adding to your workload

Accelerate your NGS library prep from start to finish with our automation-friendly solutions. By pairing robust reagents with optimized protocols, you can slash hands-on time and minimize errors.

  • For your existing liquid handlers: Our expert third-party automation team offers support across a wide range of platforms for AXELIOS 1 library prep to enable your smooth implementation of methods. Roche has already demonstrated feasibility on several liquid handlers.3

  • For complete walk-away freedom: Consider working with our AVENIO Edge System team to automate your entire workflow from nucleic acid to sequencing- ready pool. Roche has demonstrated feasibility on the AVENIO Edge System.3

Learn more about our demonstrated methods

Enter an entirely new space in NGS. Learn how our novel sequencing by expansion technology could help you uncover richer insights from every sequence.

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For Research Use Only. Not for use in diagnostic procedures. AXELIOS is a trademark of Roche.

References

  1. Chen C, et al. Single-Cell Whole Genome Analyses by Linear Amplification via Transposon Insertion (LIANTI). Science. 2017; 356(6334): 189–194.
  2. Jia H, et al. Chasing Sequencing Perfection: Marching Toward Higher Accuracy and Lower Costs. Genomics, Proteomics & Bioinformatics. 2024; 22(2): qzae024.
  3. F. Hoffmann-La Roche Ltd. Data on file.