Platelet function testing with best-in-class predictivity
Blood platelets play a pivotal role in physiological hemostasis, but also in the development of arterial thrombosis (myocardial infarction and stroke).
Platelet function testing is utilized in the analysis of inherited and acquired platelet function disorders that may cause a transient or permanent bleeding tendency. The Multiplate analyzer can detect platelet dysfunction and thus aid in the therapeutic management of such patients.
It can also be used for monitoring of antiplatelet drugs where both compliance and drug effectiveness are key issues. It was shown with Multiplate results1 that up to 20% of patients do not respond adequately to clopidogrel treatment. These patients have a 5 – 10 fold increased risk of stent thrombosis, stroke and myocardial infarction1- 4 following percutaneous coronary interventions. Multiplate delivers best-inclass predictivity 5 and evidence is available demonstrating that Multiplate guided antiplatelet therapy has the potential to improve patient outcome.6-8
The Multiplate analyzer also plays a role in the analysis of platelet function in anesthesia and intensive care, where platelet dysfunction can lead to severe bleeding complications. The detection or exclusion of platelet dysfunction before invasive procedures or in bleeding patients can aid the risk stratification and management in these situations.9-10
1 Sibbing, D. et al. (2009). J Am Coll Cardiol. Mar 10;53(10):849-56.
2 Sibbing, D. et al. (2010). Thromb Haemost. Jan;103(1):151-9.
3 Schulz, S. et al. (2010). Am Heart J. Aug; 160(2):355-61.
4 Siller-Matula, J.M. et al. (2010). J Thromb Haemost. Feb; 8(2):351-9.
5 Bonello, L. et al. (2010). J Am Coll Cardiol. Sep 14;56(12):919-33.
6 Siller-Matula, J.M. et al. (2013). Int J Cardiol. Sep 1; 167(5):2018-2023.
7 Sibbing, D. et al. (2012). J Am Coll Cardiol. 59; E265.
8 Aradi et al. (2013). J Am Coll Cardiol. 61(10): E1922.
9 Ranucci, M. et al. (2011). Ann Thorac Surg. Jan; 91(1):123-9.
10 Weber, C.F. et al. (2012). Anesthesiology, Sep; 117(3):531-47.
Cost-effective therapies :
- in cardiac surgery10
- in coronary interventions11
Best predictivity :
- for stratification of bleeding risk in surgical procedures
- for tailored anti-platelet therapy
Fast and easy assessment :
- of platelet function from small volumes of whole blood
Consistent results :
- Using standardized reagents and procedures
Medical momentum :
- More than 400 Medline publications, consensus papers with Multiplate and published guidelines for PFT
10 Weber, C.F. et al. (2012). Anesthesiology, Sep; 117(3):531-47.
11 Straub, N. et al. (2013). Thromb Haemost. Oct 24; 111(2).
- High throughput: 30 tests/hour
- Sample volume: only 300 μL per analysis
- Fast turn-around time: 10 min/test
- ADPtest: ADP induced platelet activation sensitive to clopidogrel, prasugrel and other ADP
receptor antagonists - ASPItest: Cyclooxygenase dependent aggregation (using arachidonic acid) sensitive to
Aspirin®, NSAIDs and other inhibitors of platelet cyclooxygenase - COLtest: Collagen induced aggregation
- RISTOtest: vWF and GpIb dependent aggregation (using ristocetin)
- TRAPtest: Platelet stimulation via the thrombin receptor (using TRAP-6), sensitive to IIbIIIa
receptor antagonists - Prostaglandin E1 reagent: For the assessment of ADPtest HS (high sensitivity). For the assessment of positive
(i.e. abnormal) controls of the ADPtest - ASA reagent: Inhibitor of cyclooxygenase. Addition of ASA reagent to the blood sample leads to
reduced aggregation responses in ASPItest and COLtest - GpIIb/IIIa antagonist reagent: Inhibitor of the platelet GpIIb/IIIa receptor. Addition to a blood sample leads to
strongly reduced aggregation in the TRAPtest - Hirudin blood tubes: Anticoagulant for platelet function analysis with physiological calcium
concentrations - Liquid control set: Quality control for electrical signal in impedance aggregometry based on the
analysis of an artificial liquid control material