HyperCap Workflow v2.0 Brochure
An integrated approach to sample preparation, for applications such as targeted sequencing, encompassing all the steps required to convert a sample to a sequencing-ready library is beneficial in your research considering the precious nature of NGS samples. Therefore, we offer Roche Sample Prep Solutions, from sample collection to library quantification for different sample types and sequencing applications that are proven, simple and complete.
HyperCap products provide an integrated next-generation sequencing (NGS) sample preparation solution that combines all reagents and accessories required for library preparation and target enrichment in a single workflow. The HyperCap Workflow v2.0 integrates high-efficiency KAPA Library Preparation Kits and KAPA Dual-Indexed Adapters with performance-optimized SeqCap® EZ probes and Roche Universal Blocking Oligo (UBO) Kit. The HyperCap Workflow v2.0 is an improved and streamlined version of our SeqCap EZ HyperCap Workflow, with the convenience of ordering and support from a single vendor, and is automation friendly.
KAPA HyperPrep and KAPA HyperPlus Library Preparation Kits are novel one-tube chemistry with optimally formulated enzymes selected through our directed evolution technology. They enable higher yields of adapter-ligated library and lower amplification bias resulting in higher library diversity, lower duplication rates and more uniform coverage compared to other products available in the market.*
SeqCap EZ probes use an innovative single-tube solution-based hybridization capture technology that enables enrichment of any genomic region of interest. They are built upon an optimized design algorithm and manufactured with high probe density and demonstrate uniform and higher depth of coverage..
KAPA Dual-Indexed Adapters enable greater multiplexibility compared to existing options by utilizing up to 96 sample barcodes.
Roche Universal Blocking Oligo Kits eliminate the need for adapter-matched blocking oligos, affording a simple and faster workflow.
Jennfier Pavlica, Applications Team Manager of Roche Sequencing and Life Sciences, presents Roche Sample Prep Solutions: HyperCap Workflow for Genetic Research
# | Step | SeqCap EZ v5.1 | HyperCap v1.0 | HyperCap v2.0 |
1 | Library construction chemistry | KAPA HTP/LTP Kit | KAPA HyperPrep or HyperPlus Kit | KAPA HyperPrep or HyperPlus Kit; SeqCap Adapter A/B Kits, KAPA Dual-Indexed (96) Adapters |
2 | DNA binding to SPRI beads (cleanups) | 15 min | 5 min | 5 min |
3 | Hybridization setup | Dry-down | Bead-based | Bead-based; UBO |
4 | Volume of capture beads | 100 µL | 50 µL |
50 µL |
5 | Vortex during capture | Yes | No | No |
6 | Bead capture time | 45 min | 15 min | 15 min |
7 | Bead wash volume (tube) | 200 µL (1.5 mL tube) | 200 µL (PCR tube/plate) | 200 µL (PCR tube/plate) |
8 | Bead wash parameters | Vortex mixer or water bath needed | No vortex or water bath required | No prolonged vortexing or water bath required |
9 | Post-capture LM-PCR | 2 x 50 µL reactions, 14 cycles | Single 50 µL reaction, 14 cycles | Single 50 µL reaction, 14 cycles |
HyperCap Workflow v2.0 Brochure
Roche UBO Kit Brochure
Automation in regards to the HyperCap Workflow
Instructions for product use and other product-related technical documents
Kit Code |
Roche Catalog # | Description | Kit Size (Reaction) | How to Buy |
---|---|---|---|---|
KK8510 | 7962380001 |
KAPA HyperPlus Kit, With Library Amplification |
8 | Contact Us |
KK8512 |
7962401001 |
KAPA HyperPlus Kit,With Library Amplification |
24 | Contact Us |
KK8514 |
7962428001 |
KAPA HyperPlus Kit,With Library Amplification |
96 | Contact Us |
KK8501 |
7962339001 |
KAPA HyperPrep Kit, Without amplification module |
8 | Contact Us |
KK8503 |
7962355001 |
KAPA HyperPrep Kit, Without amplification module |
24 | Contact Us |
KK8505 |
7962371001 |
KAPA HyperPrep Kit, Without amplification module |
96 | Contact Us |
KK8500 |
7962312001 |
KAPA HyperPrep Kit, With KAPA Library Amplification Primer Mix (10X) |
8 | Contact Us |
KK8502 |
7962347001 |
KAPA HyperPrep Kit, With KAPA Library Amplification Primer Mix (10X) |
24 | Contact Us |
KK8504 |
7962363001 |
KAPA HyperPrep Kit, With KAPA Library Amplification Primer Mix (10X) |
96 | Contact Us |
Kit Code |
Roche Catalog # | Description | Kit Size (Reaction) | How to Buy |
---|---|---|---|---|
N/A | 08286370001 |
HyperCap Target Enrichment Kit |
24 | Contact Us |
N/A | 08286345001 |
HyperCap Target Enrichment Kit |
96 | Contact Us |
Kit Code |
Roche Catalog # | Description | Kit Size (Reaction) | How to Buy |
---|---|---|---|---|
KK8722 | 8278555702 |
KAPA Dual-Indexed Adapter Kit, (15 µM) |
96 adapters x 20 µl each |
Contact Us |
N/A | 7141530001 |
SeqCap Adapter Kit A |
96 | Contact Us |
N/A | 7141548001 |
SeqCap Adapter Kit B |
96 | Contact Us |
N/A |
8286418001 |
HyperCap Bead Kit |
24 | Contact Us |
N/A |
8286400001 |
HyperCap Bead Kit |
96 | Contact Us |
N/A |
8286396001 |
HyperCap Universal Blocking Oligo Kit |
24 | Contact Us |
N/A |
8286388001 |
HyperCap Universal Blocking Oligo Kit |
96 | Contact Us |
TaKit Code |
Roche Catalog # | Description | Kit Size (Reaction) | How to Buy |
---|---|---|---|---|
N/A | Varies | SeqCap EZ Prime Choice Probes |
Varies |
Contact Us |
N/A | Varies |
SeqCap EZ Prime Choice XL Probes |
Varies |
Contact Us |
N/A | Varies |
SeqCap EZ Prime Developer Probes |
Varies |
Contact Us |
N/A |
Varies |
SeqCap EZ Choice Probes |
Varies |
Contact Us |
N/A |
Varies |
SeqCap EZ Choice XL Probes |
Varies |
Contact Us |
N/A |
Varies |
SeqCap EZ Developer Probes |
Varies |
Contact Us |
N/A |
Varies |
SeqCap EZ Medexome Probes |
Varies |
Contact Us |