KAPA HiFi Kits are a family of kits that all contain the same core, engineered KAPA HiFi DNA Polymerase enzyme, formulated for both NGS and PCR applications. KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with ultra-high fidelity, high efficiency and low amplification bias. This results in lower duplication rates and improved coverage of GC- and AT-rich regions, promoters, low-complexity and other challenging regions. These kits are offered in an easy-to-use ReadyMix or component format, to provide flexibility and convenience.
Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners
Roche Sequencing Solutions offers a family of KAPA HiFi Kits, containing the same core, engineered KAPA HiFi DNA Polymerase enzyme, formulated for both NGS and PCR applications. These kits are available in different formats and formulations, to provide flexibility and convenience across a broad range of applications.
The table below describes the specifications for the KAPA HiFi Kits:
KAPA HiFi PCR Kit | KAPA HiFi HotStart PCR Kit | KAPA HiFI HotStart ReadyMix PCR Kit | KAPA HiFi HotStart Library Amplification Kit | KAPA HiFi HotStart Library Amplification Kit with Primer Mix | KAPA HiFi HotStart Real-Time Library Amplification Kit | |
---|---|---|---|---|---|---|
HotStart Enzyme | No | Yes | Yes | Yes | Yes | Yes |
Format | Components | Components | ReadyMix | ReadyMix | ReadyMix with Primer Mix | ReadyMix |
Kit Contents | KAPA HiFi enzyme KAPA HiFi buffers Magnesium Chloride dNTPs |
KAPA HiFi HotStart enzyme KAPA HiFi buffers Magnesium Chloride dNTPs |
KAPA HiFi HotStart ReadyMix | KAPA HiFi HotStart ReadyMix |
KAPA HiFi HotStart ReadyMix Amplification Primer Mix |
KAPA HiFi HotStart ReadyMix Fluorescein Standards |
Recommended Applications |
PCR applications requiring flexibility and/or optimization | PCR applications requiring a HotStart enzyme and flexibility and/or optimization | Routine use in established PCR and NGS assays | Routine NGS library amplification on any platform using user-supplied primers | Routine NGS library amplification for Illumina® platforms | NGS library amplification for Illumina® platforms that require careful control |
Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners
Instructions for product use and other product-related technical documents
Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners
Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche. Other product names and trademarks are the property of their respective owners