The KAPA HiFi Uracil+ Kit forms part of the family of KAPA HiFi Kits, where KAPA HiFi HotStart Uracil+ DNA Polymerase is a modified version of KAPA HiFi HotStart DNA Polymerase that is engineered to tolerate uracil residues in bisulfite-treated DNA. Bisulfite treatment of DNA results in the conversion of unmethylated cytosine residues into uracil, while the methylated residues are left unmodified. Enzymes used for library amplification in bisulfite-sequencing workflows must be able to read through uracil residues and tolerate low concentrations of AT-rich DNA. Traditional high-fidelity DNA polymerases are typically not suitable for this type of application as the enzymes stall when a uracil is encountered.
KAPA HiFi HotStart Uracil+ DNA Polymerase has been developed to read through uracil residues and other modified bases, while still retaining the performance benefits of KAPA HiFi HotStart DNA Polymerase, including ultra-high fidelity, high efficiency and low-bias amplification. This results in lower duplication rates and improved coverage of challenging regions.
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Roche Sequencing Solutions offers KAPA HiFi Uracil+ Kits, containing the engineered KAPA HiFi HotStart Uracil+ DNA Polymerase enzyme, formulated in a Readymix format. These kits form part of the family of KAPA HiFi Kits, offering the same benefits, but evolved for the amplification of uracil and modified residues.
The table below describes the specifications for the KAPA HiFi HotStart Uracil+ ReadyMix Kits:
KAPA HiFi HotStart Uracil+ ReadyMix Kit | |
---|---|
HotStart Enzyme | Yes |
Format | ReadyMix |
Kit Contents | KAPA HiFi HotStart Uracil+ ReadyMix |
Recommended Applications | PCR and NGS applications requiring amplification of uracil or modified residues e.g. bisulphite-converted library amplification |
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Instructions for product use and other product-related technical documents
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.