KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of next-generation sequencing (NGS) libraries prepared for sequencing on Illumina and IonTorrent platforms. Kits include KAPA SYBR FAST qPCR Master Mix (formulated with different passive reference dyes for different qPCR instruments), a platform-specific library quantification primer premix, and a pre-diluted set of DNA standards. Refer to the Instrument Compatibility Chart for guidance on compatible platforms.
KAPA Library Quantification Kits contain KAPA SYBR FAST DNA Polymerase, which was engineered through our directed evolution technologyto amplify diverse DNA fragments with similar efficiency. This is important for the amplification of heterogeneous populations such as NGS libraries. Library quantification kits containing wild-type DNA polymerases only count “easy” library molecules.*
Six pre-diluted DNA Standards and appropriately diluted NGS libraries are amplified using platform-specific qPCR primers that target adapter sequences. The average Cq value for each DNA Standard is plotted against its known concentration to generate a standard curve. The standard curve is used to convert the average Cq values for diluted libraries to concentration, from which the working concentration of each library is calculated.
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Kits can be stored at -20˚C.
Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.
Spec | Description |
---|---|
Compatible Platforms | Illumina HiSeq, NextSeq, MiSeq, and GAIIx Ion Torrent Proton and PGM 454 GS FLX+ and GS Junior SOLiD 5500 series |
Library Type |
Any |
Starting Material |
Any NGS library ready for cluster amplification or emPCR |
Standard curve concentration range |
20–0.0002 pM for Illumina libraries 83–0.00083 pM for Ion Torrent and 454 libraries 10–0.0001 pg/µL for SOLiD libraries |
Sequencing Applications |
Whole Genome Sequencing, Whole Exome Sequencing, Targeted Sequencing (custom panels), RNA-Seq, ChIP-Seq, Amplicon Sequencing, Methyl-Seq |
Kit code | 10X Illumina Primer Premix |
2X KAPA SYBR FAST qPCR Master Mix (universal) |
50X KAPA SYBR FAST ROX High |
50X KAPA SYBR FAST ROX Low |
2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye) |
2X KAPA SYBR FAST qPCR Master Mix (ROX Low) |
Illumina DNA Standards 1-6 | |
---|---|---|---|---|---|---|---|---|
Complete Kit (universal) |
KK4824 | X | X | X | X | X | ||
Complete Kit (with equipment-specific dye) |
KK4835 KK4844 KK4854 |
X | X | X | ||||
Complete Kit (ROX Low) |
KK4873
|
X | X | X | ||||
qPCR MasterMix (universal) and Primer Premix only | KK4923 | X | X | X | X | |||
qPCR MasterMix (with equipment-specific reference dye) and Primer Premix only |
KK4933 KK4943 KK4953 |
X | X | |||||
qPCR MasterMix (ROX Low) and Primer Premix only |
KK4973 | X | X |
Kit Code | 10X Illumina Primer Premix |
2X KAPA SYBR FAST qPCR Master Mix (universal) |
50X KAPA SYBR FAST ROX High |
50X KAPA SYBR FAST ROX Low |
2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye) |
2X KAPA SYBR FAST qPCR Master Mix (ROX Low) |
Illumina DNA Standards 1-6 | |
---|---|---|---|---|---|---|---|---|
Complete Kit (universal) |
KK4827 | X | vX | X | X | X | ||
Complete Kitq (with equipment-specific dye) |
KK4838 KK4847 KK4857 |
X |
X |
X |
||||
Complete Kit (ROX Low) |
KK4874
|
X |
X |
X |
||||
qPCR MasterMix (universal) and Primer Premix only | KK4924 | X | X | X | X | |||
qPCR MasterMix (with equipment-specific reference dye) and Primer Premix only |
KK4934 KK4944 KK4954 |
X |
X |
|||||
qPCR MasterMix (ROX Low) and Primer Premix only |
KK4974 | X |
X |
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Instructions for product use and other product-related technical documents
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.