The KAPA RNA HyperPrep Kit with RiboErase (HMR) targets and depletes rRNA using DNA probes and RNase H to provide improved coverage of transcripts of interest, including precursor mRNAs and important regulatory species, such as noncoding RNAs. Sequencing of rRNA-depleted total RNA samples provides a more comprehensive representation of the whole transcriptome. This kit is suitable for total RNA input from human, mouse and rat species only.
• rRNA depletion and purification in ~2.5 hours*
• Robust performance across different sample types and input amounts
• High success rates with low-input and degraded samples*
• Qualified automation methods
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Enzymes and buffers for rRNA depletion, cDNA synthesis, and library preparation can be stored at 4°C. (US only)
Kits contain Hybridization Buffer, Hybridization Oligos (HMR), Depletion Buffer, RNase H, DNase Buffer, DNase, Fragment, Prime and Elute Buffer (2X), 1stStrand Synthesis Buffer, KAPA Script, 2nd Marking Buffer, 2nd Strand Synthesis & A-Tailing Enzyme Mix, Ligation Buffer, DNA Ligase, PEG/NaCl Solution, KAPA Pure Beads, Library Amplification Primer Mix (10X), and KAPA HiFi HotStart ReadyMix (2X).
Spec |
Description |
---|---|
Compatibility Platform |
Illumina HiSeq, NextSeq, and MiSeq |
Library Type |
RNA |
Starting Material | High-quality and degraded total RNA |
Starting Material |
25 ng – 1 µg |
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.
Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.