MagNA Pure FFPET Buffer Set

Overview

The MagNA Pure FFPET Buffer Set is a general sample pretreatment buffer to enable nucleic acid purification in automated and semi-automated workflows. When used with MagNA Pure 24 System, it gives an exclusive feature for a fully automated FFPET sample extraction workflow.  

Features and Benefits

The MagNA Pure FFPET Buffer Set features:

  • Elimination of hazardous and odor for deparaffinization
  • Deparaffinization and lysis of formalin-fixed, paraffin-embedded tissue samples in automated and manual nucleic acid isolation workflows
  • Designed as a generic buffer and compatible with pre-optimized protocols on MagNA Pure Systems
  • Simplicity with only 3 components for easy setup
  • Flexibility across a broader range of genomic applications including real-time PCR, and next-generation sequencing

Contents

The MagNA Pure FFPET Buffer Set contains all components needed for FFPET deparaffinization and lysis with no need to order extra products. The Buffer Set is used upstream of extraction, similar to other sample pre-treatment buffers.

  • Deparaffinization reagent
  • Lysis Buffer
  • Isopropanol

Application

The MagNA Pure FFPET Buffer Set is a set of reagents intended for general laboratory use to enable the isolation of DNA and RNA from FFPET samples. The buffer is compatible with manual or automated nucleic acid extraction, such as the MagNA Pure 96 and the MagNA Pure 24 Systems. FFPET extraction are the targets of interest in downstream applications of Oncology study.

Detail

When used with the MagNA Pure 24 System, the MagNA Pure FFPET Buffer Set is used on-board as an pre-treatment reagents to support nucleic acid purification with the MagNA Pure 24 Total NA Isolation Kit (07 658 036 001) and MagNA Pure 24 DNA FFPET 1000 protocol.  When used with the MagNA Pure 96 System, the buffer is used as an external pre-treatment reagents off-board. In the following workflow on MagNA Pure 96 Instrument, MagNA Pure 96 DNA and Viral NA Small Volume Kit (06 543 588 001) for the DNA FFPE SV protocol or MagNA Pure 96 Cellular RNA Large Volume Kit (05 467 535 001) for RNA Tissue FFPE LV protocol.

The MagNA Pure 24 FFPET workflow, listed below.

 Protocol for FFPET on MagNA Pure 24 System

 FFPET starting volume (µm), elution volume (µL)

 DNA FFPET 1000
  • Start: up to 6 mm3 formalin-fixed paraffin-embedded tissue, corresponding to 6 FFPET sections of 4 or 5 µm
  • Elution: 50 µL or 100 µL

The MagNA Pure 96 FFPET workflow, listed below.

 Protocols for FFPET on MagNA Pure 96 System  FFPET starting volume (µm), elution volume (µL)
 DNA FFPET SV
  • Start: 2 sections of 5 µm formalin-fixed paraffin-embedded tissue
  • Elution: 50 µL or 100 µL

 RNA Tissue FFPE LV

  • Start: 2 sections of 5 µm formalin-fixed paraffin-embedded tissue
  • Elution: 50 µL or 100 µL