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Discontinued SeqCap Portfolio

SeqCap Epi Developer Probes has been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.

Overview

SeqCap Epi Developer Probes enable the targeting of selected genomic regions (up to 210 Mb) for epigenetic studies from bisulfite-treated genomic DNA in a single workflow. Built on the Roche proprietary probe design and manufacturing technologies, SeqCap Epi Developer Probes allow for complete customization for targeting both strands of any organisms’ bisulfite-treated genomic DNA.

Features and benefits of the SeqCap Epi Developer Probes
  •  Efficient discovery of differential methylation
  •  Optimized design and workflow to effectively conserve epigenome complexity
  •  Maximized epigenetic discovery while minimizing sequencing costs
Product Highlights

High depth of coverage

  •  Targeting epigenomic region of interest with a custom enrichment solution for bisulfite sequencing
  •  Better depth of coverage and higher resolution than whole genome bisulfite sequencing (WGBS)1
Epi_Developer_Figure1.png

Coverage per base comparison across three sequencing depths. The 80.5 Mb SeqCap Epi CpGiant Probes design was sequenced using Illumina 2x100 bp reads, subsampled to calculate gigabases of sequencing needed per sample for 1x, 10x and 30x mean coverage depths. Based on these results 4 samples could be sequenced in one lane of a HiSeq 2000 to achieve 30x mean coverage.

 

Epi_Developer_Figure1.png

Coverage per base comparison across three sequencing depths. The 80.5 Mb SeqCap Epi CpGiant Probes design was sequenced using Illumina 2x100 bp reads, subsampled to calculate gigabases of sequencing needed per sample for 1x, 10x and 30x mean coverage depths. Based on these results 4 samples could be sequenced in one lane of a HiSeq 2000 to achieve 30x mean coverage.

 

Observation of methylation changes on both DNA strands

  • Advanced design algorithm coupled with the ability to manufacture over 2.1 million long oligonucleotide probes provides superior target enrichment performance
  • Enables observation of methylation changes on both strands while preserving the ability to decipher  SNPs vs. methylation events
  • Sample input amounts of just 1µg*
Custom Designs

NimbleDesign, our free online tool, enables you to quickly and easily design custom SeqCap EZ Choice, SeqCap EZ Choice XL or HEAT-Seq® Choice Designs for human and non-human research applications. Learn more about the NimbleDesign software for custom target enrichment designs.

References

1. Fu et al. 2010, PLOS One. High-resolution genotyping via whole genome hybridizations to microarrays containing long oligonucleotide probes.

*Data on file.

Research Use Only. Not for use in diagnostic procedures.
SEQCAP, HEAT-SEQ and NIMBLEDESIGN are trademarks of Roche. All other names and trademarks are the property of their respective owners.

Discontinued SeqCap Portfolio

SeqCap Epi Developer Probes has been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.


Technical Documents

Instructions for product use and other product-related technical documents

Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. Other names and trademarks are the property of their respective owners.

Discontinued SeqCap Portfolio

SeqCap Epi Developer Probes has been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.

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Description Catalog Number Pack Size How to Buy?
SeqCap Epi Developer S Enrichment Kits 07139071001 12 Reactions Not Available
SeqCap Epi Developer S Enrichment Kits 07139080001 48 Reactions Not Available
SeqCap Epi Developer S Enrichment Kits 07139098001 384 Reactions Not Available
SeqCap Epi Developer M Enrichment Kits 07139101001 12 Reactions Not Available
SeqCap Epi Developer M Enrichment Kits 07139128001 48 Reactions Not Available
SeqCap Epi Developer M Enrichment Kits 07139578001 384 Reactions Not Available
SeqCap Epi Developer L Enrichment Kits 07139586001 12 Reactions Not Available
SeqCap Epi Developer L Enrichment Kits 07139594001 48 Reactions Not Available
SeqCap Epi Developer L Enrichment Kits 07139608001 384 Reactions Not Available
SeqCap Epi Developer XL Enrichment Kits 07139624001 12 Reactions Not Available
SeqCap Epi Developer XL Enrichment Kits 07139659001 48 Reactions Not Available
SeqCap Epi Developer XL Enrichment Kits 07139667001 384 Reactions Not Available

Reagents and accessories are available separately.

Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. Other names and trademarks are the property of their respective owners.