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SeqCap EZ Choice Probes

Overview

SeqCap® EZ Choice Probes allow for enrichment of genomic regions of interest up to 7 Mb for human resequencing studies with an automation-friendly workflow. SeqCap® EZ Choice Probes are replaced by SeqCap® EZ Prime Choice Probes, which provide improved GC coverage compared to SeqCap EZ Choice Probes.

Benefits of SeqCap EZ Choice Enrichment Kits
  • Capture up to 7 Mb custom regions with a single design utilizing up to 2.1 million probes
  • Achieve uniform and high performance capture and save on sequencing costs
  • Capture more target regions to identify more variants compared to other enrichment methods*
  • Obtain higher coverage of target regions with our advanced repeat masking methods
  • Combine with KAPA's library preparation products to create the HyperCap Workflow v2.0 for a fully integrated sample preparation solution.
Product Highlights

Capture more target regions compared to competitor kits

WebGraphs_SeqCapEZ_choice_Figure1.png

Capture more target regions. Design comparison of SeqCap EZ Choice Library versus Agilent SureSelect Kit for a 500 kb target on chr11 (61-61.5 Mb). SeqCap EZ Choice probes target 20% more of the target region (372 kb vs. 222 kb), as illustrated by an increase in number and coverage black horizontal bars, denoting more coverage of the target regions. Note: The SeqCap EZ Choice design was created using Roche standard design parameters; the SureSelect design was created using Agilent eArray online design tool using default design parameters.

WebGraphs_SeqCapEZ_choice_Figure1.png

Capture more target regions. Design comparison of SeqCap EZ Choice Library versus Agilent SureSelect Kit for a 500 kb target on chr11 (61-61.5 Mb). SeqCap EZ Choice probes target 20% more of the target region (372 kb vs. 222 kb), as illustrated by an increase in number and coverage black horizontal bars, denoting more coverage of the target regions. Note: The SeqCap EZ Choice design was created using Roche standard design parameters; the SureSelect design was created using Agilent eArray online design tool using default design parameters.

Less sequencing and more coverage per base with SeqCap EZ Choice

WebGraphs_SeqCapEZ_choice_Figure2.png

Coverage per base comparison across three sequencing depths. The SeqCap EZ Choice Comprehensive Cancer Design was sequenced using Illumina 2x76 bp reads, subsampled to calculate Gb of sequencing needed per sample for 75x, 100x and 150x coverage depths.

WebGraphs_SeqCapEZ_choice_Figure2.png

Coverage per base comparison across three sequencing depths. The SeqCap EZ Choice Comprehensive Cancer Design was sequenced using Illumina 2x76 bp reads, subsampled to calculate Gb of sequencing needed per sample for 75x, 100x and 150x coverage depths.

Minimize sequencing costs through efficient multiplexing

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Sample Multiplex Capabilities
Coverage Illumina HiSeq (34 Gb*) Illumina MiSeq (4 Gb*)
75x 14 Samples 2 Samples
100x 12 Samples 1 Sample
150x 7 Samples -
*Estimates for sequencing throughput per lane are based on Manufacturer's specifications
Design your own targets

NimbleDesign, our free online tool, enables you to quickly and easily design custom SeqCap EZ Prime Choice, SeqCap EZ Prime Developer Probes, SeqCap EZ Prime Choice XL or HEAT-Seq® Choice Designs for human and non-human research applications. Learn more about the NimbleDesign software for custom target enrichment designs.

Sample throughput multiplexing. Illustrated at 75x, 100x, and 150x coverage rates with 90% bases covered using Illumina 2x76 bp reads on either a HiSeq or MiSeq instrument.

  *Data on file.

Research Use Only. Not for use in diagnostic procedures.
SEQCAP, HEAT-SEQ, NIMBLEDESIGN and HYPERCAP are trademarks of Roche. All other product names and trademarks are the property of their respective owners.


Technical Documents

Instructions for product use and other product-related technical documents

Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. All other product names and trademarks are the property of their respective owners.

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Description Target Regions Catalog Number
 Pack Size How to Buy
SeqCap EZ Choice
 up to 7Mb human genomic regions
 06740251001
 4 Reactions Contact Us
SeqCap EZ Choice
 up to 7Mb human genomic regions
 06266282001
 12 Reactions
 Contact Us
SeqCap EZ Choice
 up to 7Mb human genomic regions
 06266304001
 24 Reactions
 Contact Us
SeqCap EZ Choice
 up to 7Mb human genomic regions
 06266312001
 48 Reactions
 Contact Us
SeqCap EZ Choice
 up to 7Mb human genomic regions
 06266339001
 96 Reactions
 Contact Us
SeqCap EZ Choice
 up to 7Mb human genomic regions
 06266347001
 384 Reactions
 Contact Us
SeqCap EZ Choice
 up to 7Mb human genomic regions
 06266355001
 960 Reactions
 Contact Us

Reagents and accessories are available separately.

Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. All other product names and trademarks are the property of their respective owners.