SeqCap EZ MedExome Probes has been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome, as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.
An integrated approach to sample preparation, for applications such as whole exome sequencing, encompassing all the steps required to convert a sample to a sequencing-ready library is beneficial in your research considering the precious nature of NGS samples. Therefore, we offer Roche Sample Prep Solutions, from sample collection to library quantification for different sample types and sequencing applications that are proven, complete and simple.
The SeqCap® EZ MedExome Probes target the human exome (HRCh38/hg38, coordinates for hg19 annotation are also available) with enhanced exon coverage for medically relevant genes. The MedExome design has been extensively optimized using empirical rebalancing to increase coverage in low coverage regions for a more uniform representation of the human exome.
The design and annotation files provide information about genomic regions covered by the capture probes and the genes included in these regions.
The SeqCap EZ MedExome Probes was designed to cover exons from from the following annotation sources:CCDS 17, RefSeq CDS August 2014, Ensembl 76 CDS (biotype filtered), VEGA 56 CDS, GENCODE 20 CDS and miRBase 21. The design also includes coverage for regions defined as medically relevant, including GeneTests CDS (excluding mitochondrial genes), ClinVar, coding sequence from the set of ~4600 genes identified by the consortium of the Emory Genetics Lab, Harvard Laboratory of Molecular Medicine, and Children's Hospital of Philadelphia (CHOP) and additional regions deemed as medically relevant based on customer input. The genome assembly coordinates are based on the UCSC human genome build hg38, though hg19 files are also provided.
The total design size is 47Mb of capture target space; the capture targets BED file shows the capture probe footprint. The UCSC Browser BED files are provided for upload as a custom track. All design files are provided in the original hg38 genome assembly and also converted to hg19 genome assembly. Conversion was performed using the NCBI Genome Remapping Service.
Download Design Files for the SeqCap EZ MedExome Probes
Download Design Files for the SeqCap EZ MedExome Probes used with mitochondrial genome design.
Demo data is available for evaluation. Please contact Roche representative for your country.
Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. All other product names and trademarks are the property of their respective owners.
SeqCap EZ MedExome Probes has been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome, as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.
Instructions for product use and other product-related technical documents
Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. All other product names and trademarks are the property of their respective owners.
SeqCap EZ MedExome Probes has been discontinued. Our new KAPA Target Enrichment Portfolio is now available and contains our new KAPA HyperExome, as well as our new custom probes, KAPA HyperChoice and KAPA HyperExplore. For additional questions, please contact your regional sales representative.
Product | Catalog Number |
Pack Size | How to Buy |
---|---|---|---|
SeqCap EZ MedExome Enrichment Kit |
07676581001 |
4 Reactions |
Not Available |
SeqCap EZ MedExome Enrichment Kit |
07681330001 |
48 Reactions |
Not Available |
SeqCap EZ MedExome Enrichment Kit |
07681348001 |
384 Reactions |
Not Available |
SeqCap EZ MedExome Enrichment PLUS Kit |
07681364001 |
48 Reactions |
Not Available |
Reagents and accessories are available separately.
Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. All other product names and trademarks are the property of their respective owners.