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Overview

SeqCap® EZ Prime Exome targets the GRCh38/hg38 genome and has a compact capture target of 37 Mb. The 2.1 million overlapping probes are tiling 98.1% of the coding regions based on the latest CCDS Rel.20.1 To enable you to easily track samples throughout the workflow, the design also includes a unique sample-intrinsic identification method with 340 informative SNPs. SeqCap EZ Prime Exome is available as part of the Roche Sequencing Solutions’ Design Share portfolio

Features and Benefits of SeqCap EZ Exome
  • Obtain uniform and comprehensive exome coverage (96.3% of the primary target at 20x depth with only 40M high-quality reads) to minimize sequencing costs
  • Utilize a complete solution from a single vendor with the HyperCap® Workflow, an optimized protocol delivering consistent performance from sample prep to sequencing in 1.5 days
  • Track samples with SNP targets included in the design to eliminate the need to spike in a sample identification panel so you can have increased confidence from sample traceability
Product Highlights

High sequencing efficiency

  • Optimized to deliver both high uniformity and high on-target rate
  • Minimizes the need for additional confirmation with at least 96% coverage of target bases at ≥20x with 20 M 2 x 100 bp read pairs from only 100 ng of input DNA
performance-metrics-1a.jpg.png

SeqCap EZ Prime Exome exhibits high on-target rates with high depth of coverage. Samples were 8-plexed and processed in duplicates using the HyperCap protocol (HyperCap User’s Guide v2.1) and a simple exome-specific modification outlined in the technical note “How to …. Increase sequencing efficiency with the SeqCap EZ Prime Exome”. The 8-plex pools were sequenced on a HiSeq 2500 System with 2 x 101 bp reads using a High Output v4 Kit. The numbers across the bars represent the average of the samples tested.

performance-metrics-1a.jpg.png

SeqCap EZ Prime Exome exhibits high on-target rates with high depth of coverage. Samples were 8-plexed and processed in duplicates using the HyperCap protocol (HyperCap User’s Guide v2.1) and a simple exome-specific modification outlined in the technical note “How to …. Increase sequencing efficiency with the SeqCap EZ Prime Exome”. The 8-plex pools were sequenced on a HiSeq 2500 System with 2 x 101 bp reads using a High Output v4 Kit. The numbers across the bars represent the average of the samples tested.

Broad coverage of relevant medical-research content

  • Higher sequencing coverage of ACMG,1 ClinVar2 and medical-research relevant targets3 compared to another supplier’s exome
  • Better coverage with 27% less sequencing than another supplier’s exome
performance-metrics-1c.png

Better coverage of medical-research genes with less sequencing. SeqCap EZ Prime Exome and another exome (from Supplier I) were sequenced proportionally to their target size. At least 8 samples per exome were prepared according to manufacturers’ instructions and sequenced on a HiSeq 2500 system. For accurate comparison, same Coriell DNA samples were used in both exomes.

performance-metrics-1c.png

Better coverage of medical-research genes with less sequencing. SeqCap EZ Prime Exome and another exome (from Supplier I) were sequenced proportionally to their target size. At least 8 samples per exome were prepared according to manufacturers’ instructions and sequenced on a HiSeq 2500 system. For accurate comparison, same Coriell DNA samples were used in both exomes.

Enhanced sample tracking with SNPs for confidence in results

  • Targets 340 informative SNP positions that may be used as a sample intrinsic unique identification method, to allow sample tracking throughout the testing workflow
  • The SNP positions have been selected based on literature4 and customer feedback
  • Enables accurate and convenient sample tracking and delivers high overall SNP detection performance
performance-metrics-1b.png

Integrated sample tracking with high SNP detection performance. Samples were 8-plexed and processed in duplicates using the HyperCap Users’ Guide v2.1 protocol and a simple exome-specific modification outlined in SeqCap EZ Prime Exome technical note. The 8-plex pools were sequenced on a HiSeq 2500 system with 20 M 2x101 bp reads using a High Output v4 kit.

performance-metrics-1b.png

Integrated sample tracking with high SNP detection performance. Samples were 8-plexed and processed in duplicates using the HyperCap Users’ Guide v2.1 protocol and a simple exome-specific modification outlined in SeqCap EZ Prime Exome technical note. The 8-plex pools were sequenced on a HiSeq 2500 system with 20 M 2x101 bp reads using a High Output v4 kit.

SeqCap EZ Prime Exome is a part of the Roche Sequencing Solutions’ Design Share Portfolio. Design Share makes it easy to access pre-designed NGS panels that are developed by Roche Sequencing Solutions or in collaboration with researchers around the world.

Other panels include:

Access the other Design Share portfolio to view other exome products.

Related Products

HyperCap Workflow Products
SeqCap EZ Design Share Panels

References

  1. Kalila SS, Adelman K, Bale SJ, et al. Recommendations for reporting of secondary findings in clinical exome and genome sequencing, 2016 update (ACMG SF v2.0): a policy statement of the American College of Medical Genetics and Genomics. Genet Med. 2017 Feb; 19(2):249-255.
  2. https://www.ncbi.nlm.nih.gov/clinvar/
  3. As selected by GeneTests™.
  4. Pengelly RJ, Gibson J, Andreoletti G, et al. A SNP profiling panel for sample tracking in whole-exome sequencing studies. et al. 2013 Sep 27;5(9):89.

Research Use Only. Not for use in diagnostic procedures.
SEQCAP, KAPA and HYPERCAP are trademarks of Roche. All other product names and trademarks are the property of their respective owners.


Technical Documents

Instructions for product use and other product-related technical documents

Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. All other product names and trademarks are the property of their respective owners.

Product Type: SeqCap EZ Share Prime Choice XL
Design Name: 170907_HG38_CCDS1X_REZ_HX1
Internal Reference #: 4000035460

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Description Catalog Number
 Pack Size How to Buy
SeqCap EZ  Share Prime Choice XL
 08333092001 24 Reactions Contact Us
SeqCap EZ  Share Prime Choice XL
 08333114001 96 Reactions
 Contact Us
SeqCap EZ  Share Prime Choice XL
 08333122001 384 Reactions
 Contact Us

Research Use Only. Not for use in diagnostic procedures.
SEQCAP is a trademark of Roche. All other product names and trademarks are the property of their respective owners.