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Preparing target nucleic acids for next-generation sequencing
Library construction is a first, critical step in the next generation sequencing (NGS) workflow. DNA or RNA samples are first fragmented to the appropriate size and then sequencing platform-specific adapters are added. In most cases, libraries have to be amplified before the next step in the process (target enrichment or sequencing). Each step can potentially introduce errors or biases that could significantly impact library quality and ultimately affect the quality and reliability of sequencing results. It is therefore imperative to use the highest quality reagents and optimized protocols to obtain the best sequencing libraries, lowest bias, and the highest and most uniform coverage.
Roche Sequencing Solutions offers a range of library preparation kits that delivers the highest quality sequencing libraries suitable for use in different sequencing platforms. Kits contain high-quality enzymes selected through our Directed Evolution Technology, and formulated in convenient, easy-to-use master mixes.