For in vitro diagnostic use. Others LightMix in-vitro diagnostics kit MTHFR A1298C CE-IVD IVD LightMix® in-vitro diagnostics kit MTHFR A1298C CE-IVD 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0120 Detection of the A1298C DNA variation in the MTHFR genefor use with the Roche Diagnostics LightCycler® Instruments 06 896 383 001 6 896 383 001 06896383001 6896383001 06896383001 LightMix MTHFR A1298C LightMix MTHFR A1298C 04260159330762 Reagents, kits 40-0269-64 Reagents for 64 reactions Not Available false This kit allows the detection of the Methylenetetrahydrofolate Reductase (MTHFR, OMIM: 607093) A1298C single nucleotide polymorphism (SNP) rs1801131 in genomic human DNA from a nucleic acid extract obtained from peripheral blood. MTHFR mutations may contribute to increased plasma homocysteine, which represents a risk factor for arteriosclerotic vascular disease and deep-vein thrombosis, but also for neural tube defects and unexplained, recurrent embryo losses in early pregnancy. The MTHFR A1298C gene polymorphism encodes an enzyme variant with a decreased activity. Most publications consider only the compound heterozygous constitution with allele combination A1298C and C677T as a risk factor. Patients which were found 1298 C/C homozygous or A/C heterozygous should be analyzed for the MTHFR polymorphism C677T too, for example by using the LightMix® Kit 40-0129-64. This product is intended to help the clinician to analyze the genetic background showing increased homocysteine levels or a propensity to thrombosis. Together with the results from MTHFR C677T testing it helps the clinician to analyze possible reasons that might contribute to spontaneous abortion (SA) of fetal tissues especially in early pregnancy, and neural tube deficiencies. The present test can be performed in addition or after a phenotypical assay of homocysteine levels. The kit is not intended to be the only basis for therapy decision. The patient´s mutation status should be considered alongside other disease factors. Note: The performance of the assay can be guaranteed only when used with LightCycler® Instruments (see 1.3.2 for details). en Using PCR methodology, a 269 bp fragment of the MTHFR gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® oligomer binding to the region spanning the mutation site.During the melting curve analysis the temperature is slowly increased. The probe leaves at a specific temperature (Tm) causing a fluorescence decrease. Any mismatch covered by the probe destabilizes the hybrid and lowers the Tm.In this product the probe matches the sequence of the wild type genotype and the presence of a mutation will yield a reduced Tm.Reading of the genotype results is based on the melting temperatures compared to the supplied standards. If permitted by the instrument software reading of the genotype results can be achieved by the automated genotyping module (instrument-dependent: software module 'Melt Curve Genotyping').Automated genotyping results must be reviewed by eye for deviating curves and intermediate melting point temperatures. In case that automated typing fails to report consistent genotype results, the genotype must be deduced from the melting temperatures following the criteria described in the method sheet, chapter 7. en

LightMix^® in-vitro diagnostics kit MTHFR A1298C CE-IVD

Detection of the A1298C DNA variation in the MTHFR gene
for use with the Roche Diagnostics LightCycler^® Instruments

IVD

User profile

Select your user profile

LightMix^® in-vitro diagnostics kit MTHFR A1298C CE-IVD

Overview

Ordering information

Technical documents

Compatible products

Related products

Contact us