For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Respi-BAC-1 RUO UC-TIB-Respi-BAC-1 (S.pneu/B.pert/B.para/H.inf) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000708 10 175 362 001 10175362001 UC-TIB-Respi-BAC-1 UC-TIB-Respi-BAC-1 04260159335293 Reagents, kits 192 reactions Not Available false UC-TIB-Respi-BAC-1 is an automated qualitative in vitro test for the detection of respiratory bacterial DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of Streptococcus pneumoniae (S.pneu), Bordetella pertussis / holmesii (B.pert), Bordetella parapertussis / bronchiseptica (B.para) and Haemophilus influenzae (H.inf). en Bacterial DNA is analyzed by real-time PCR, that amplifies a 75 bp long fragment of lytA gene from S.pneu analyzed with a Coumarin-labeled probe in channel 01, a 69 bp long fragment of IS481 gene from B.pert analyzed with a FAM-labeled probe in channel 02, a 80 bp long fragment of IS1001 gene from B.para analyzed with a HEX-labeled probe in channel 03 and a 137 bp long fragment of siaT gene from H.inf analyzed with a JA270 labeled probe in channel 04. The (+) Ctrl comprises plasmids with a 473 bp long synthetic fragment of lytA gene from S.pneu, a 363 bp long synthetic fragment of IS481 gene from B.pert, a 495 bp long synthetic fragment of IS1001 gene from B.para and a 400 bp long synthetic fragment of siaT gene from H.inf (Accession number: M13812 position 687 - 1160 for lytA gene from S.pneu, M28220 position 695 - 1057 for IS481 gene from B.pert, X66858 position 1 - 495 for IS1001 gene from B.para and DQ054471 position 794 - 1194 siaT gene from H.inf respectively). en The limit of detection (LoD) for UC-TIB-Respi-BAC-1 was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 μL sample volume). The study demonstrates that the UC-TIB- Respi-BAC-1 detects at least 92 genome equivalent copies or less per mL for S.pneu, 68 copies or less per mL for B.pert, 82 copies or less per mL for B.para and 105 copies or less per mL for H.inf, determined by LOGIT analysis with a hit rate of 95%. en

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