For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Respi-BAC-2 RUO UC-TIB-Respi-BAC-2 (L.pneu/C.pneu/M.pneu) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000707 10 175 389 001 10175389001 UC-TIB-Respi-BAC-2 UC-TIB-Respi-BAC-2 04260159335309 Reagents, kits 192 reactions Not Available false UC-TIB-Respi-BAC-2 is an automated qualitative in vitro test for the detection of respiratory bacterial DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of Legionella pneumophila (L.pneu), Chlamydia pneumoniae (C.pneu) and Mycoplasma pneumoniae (M.pneu). en Bacterial DNA is analyzed by real-time PCR, that amplifies a 78 bp long fragment of mip gene from L.pneu analyzed with a Coumarin-labeled probe in channel 1, a 79 bp long fragment of argR gene from C.pneu analyzed with a FAM-labeled probe in channel 2 and a 98 bp long fragment of CARDS toxin gene from M.pneu analyzed with a HEX-labeled probe in channel 3. The (+) Ctrl comprises plasmids with a 355 bp long synthetic fragment of mip gene from L.pneu, a 392 bp long synthetic fragment of argR gene from C.pneu and a 399 bp long synthetic fragment of CARDS toxin gene from M.pneu (Accession number: AF023173 position 16 - 371 for mip gene from L.pneu, LN849024 position 2711 - 3102 for argR gene from C.pneu and DQ447750 position 1367 - 1763 for CARDS toxin gene from M.pneu respectively). en The limit of detection (LoD) for UC-TIB-Respi-BAC-2 was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 μL sample volume). The study demonstrates that the UC-TIB- Respi-BAC-2 detects at least 78 genome equivalent copies or less per mL for L.pneu , 123 copies or less per mL for C.pneu and 256 copies or less per mL for M.pneu, determined by LOGIT analysis with a hit rate of 95%. en

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