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Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Background Information", "Value": "Cytokeratin (CK) 5 is an intermediate filament protein of 58-kD molecular weight within the cytokeratin family.¹ It is a type II (basic) cytokeratin.² Antibodies to this protein identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium. Epithelioid mesotheliomas are positive in most cases, but a minority of pulmonary adenocarcinomas will show focal immunoreactivity.^4,5 Most squamous cell carcinomas, some urothelial carcinomas, and undifferentiated large cell carcinomas are reactive with anti-CK^5.6 Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are also reactive with anti-CK 5.^2-4,7

1. Lersch R, Fuchs E. Sequence and expression of a type II keratin, K5, in human epidermal cells. Mol Cell Biol. 1988 Jan;8(1):486-93.
2. Moll R, Dhouailly D, Sun TT. Expression of keratin 5 as a distinctive feature of epithelial and biphasic mesotheliomas. An immunohistochemical study using monoclonal antibody AE14. Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;58(2):129-45.
3. Clarke CL, Sandle J, Parry SC, Reis-Filho JS, O’Hare MJ, Lakhani SR. Cytokeratin 5/6 in normal human breast: lack of evidence for a stem cell phenotype. J Pathol. 2004 Oct;204(2):147-52.
4. Dabbs DJ, Chivukula M, Carter G, Bhargava R. Basal phenotype of ductal carcinoma in situ: recognition and immunohistologic profile. Mod Pathol. 2006 Nov;19(11):1506-11.
5. Ordóñez NG. What are the current best immunohistochemical markers for the diagnosis of epithelioid mesothelioma? A review and update. Hum Pathol. 2007 Jan;38(1):1-16.
6. Kargi A, Gurel D, Tuna B. The diagnostic value of TTF-1, CK 5/6, and p63 immunostaining in classification of lung carcinomas. Appl Immunohistochem Mol Morphol. 2007 Dec;15(4):415-20.
7. Netto GJ, Epstein JI. Diagnostic immunohistochemistry, theranostic and genomic applications. 3rd ed. Dabbs DJ, editor. Philadelphia: Saunders Elsevier; 2010. p.595-6.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Intended Use", "Value": "Le Cytokeratin 5 (SP27) Rabbit Monoclonal Primary Antibody est destiné à un usage en laboratoire pour détecter la protéine Cytokératine-5 dans les tissus humains fi xés au formol et enrobés à la paraffi ne, colorés en immunohistochimie (IHC) qualitative sur les instruments BenchMark IHC/ISH.

Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés.

Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "Cytokeratin 5 (SP27) Rabbit Monoclonal Primary Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "Le Cytokeratin 5 (SP27) Rabbit Monoclonal Primary Antibody est destiné à un usage en laboratoire pour détecter la protéine Cytokératine-5 dans les tissus humains fi xés au formol et enrobés à la paraffi ne, colorés en immunohistochimie (IHC) qualitative sur les instruments BenchMark IHC/ISH.

Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés.

Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Product Purpose" } ] } } ] }