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}, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Upon receipt and when not in use, store at 2-8°C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Principle", "Value": "Podoplanin (D2-40) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specifi c temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "Le Podoplanin (D2-40) Mouse Monoclonal Primary Antibody est destiné à un usage en laboratoire pour détecter la glycoprotéine podoplanine dans les tissus humains fixés au formol et enrobés à la paraffine, colorés en immunohistochimie (IHC) qualitative sur les instruments BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "Podoplanin is a transmembrane mucoprotein (38 kD) recognized by the D2-40 monoclonal antibody. Podoplanin is selectively expressed in lymphatic endothelium as well as lymphangiomas, and Kaposi sarcomas. Podoplanin has also been shown to be expressed in epithelioid mesotheliomas and seminomas.^1-9

1. Ordóñez NG. Podoplanin: a novel diagnostic immunohistochemical marker. Adv Anat Pathol. 2006; 13:83-8.
2. Ordóñez N. D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma. Hum Pathol. 2005; 36:372-80.
3. Niakosari F, et al. Detection of lymphatic invasion in primary melanoma with monoclonal antibody D2-40: a new selective immunohistochemical marker of lymphatic endothelium. Arch Dermatol. 2005; 141:440-4.
4. Galambos C, et al. Identification of lymphatic endothelium in pediatric vascular tumors and malformations. Pediatr Dev Pathol. 2005; 8:181-9.
5. Fukunaga M. Expression of D2-40 in lymphatic endothelium of normal tissues and in vascular tumours. Histopathology. 2005; 46:396-402.
6. Chu AY, et al. Utility of D2-40, a novel mesothelial marker, in the diagnosis of malignant mesothelioma. Mod Pathol. 2005; 18:105-10.
7. Franke FE, et al. Hobnail hemangiomas (targetoid hemosiderotic hemangiomas) are true lymphangiomas. J Cutan Pathol. 2004; 31:362-7.
8. Fogt F, et al. Identification of lymphatic vessels in malignant, adenomatous and normal colonic mucosa using the novel immunostain D2-40. Oncol Rep. 2004; 11:47-50.
9. Kahn HJ, et al. Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi’s sarcoma and a subset of angiosarcomas. Mod Pathol. 2002; 15:434- 40.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Product Purpose", "Value": "Le Podoplanin (D2-40) Mouse Monoclonal Primary Antibody est destiné à un usage en laboratoire pour détecter la glycoprotéine podoplanine dans les tissus humains fixés au formol et enrobés à la paraffine, colorés en immunohistochimie (IHC) qualitative sur les instruments BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Product Purpose" } ] } } ] }