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2-8°C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "Le SV40 (MRQ-4) Mouse Monoclonal Primary Antibody est destiné à un usage en laboratoire pour détecter l’antigène du virus simien 40 (SV40) dans les tissus fixés au formol et enrobés à la paraffine, colorés sur les instruments VENTANA BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "SV40, Simian Virus 40, is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors. SV40 is believed to suppress the transcriptional properties of tumor-suppressing p53 in humans through the SV40 large T-antigen and SV40 small T-antigen.^1-2 It is generally assumed that large T-antigen is the major protein involved in neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumor suppressor p53, which is responsible for initiating regulated cell death (“apoptosis”), or cell cycle arrest when a cell is damaged. Anti-SV40 recognizes the large T-antigen of SV40.³ A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor. The hypothesis that SV40 might cause cancer in humans has been a particularly controversial area of research.⁴ Some research has suggested that SV40 is associated with brain tumors, bone cancers, non-Hodgkin lymphoma, and malignant mesothelioma.^1,2,5,6

1. Huang H, et al. Identification in human brain tumors of DNA sequences specific for SV40 large T antigen. Brain Pathol. 1999; 9:33-42.
2. Butel JS. Increasing evidence for involvement of SV40 in human cancer. Dis Markers. 2001; 17:167-72.
3. Gurney EG, et al. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980; 34:752-63.
4. Poulin DL, et al. Is there a role for SV40 in human cancer? J Clin Oncol. 2006; 24:4356-65.
5. Pershouse M, et al. The role of SV40 in malignant mesothelioma and other human malignancies. Inhal Toxicol. 2006; 18:995-1000.
6. Kroczynska B, et al. Crocidolite asbestos and SV40 are cocarcinogens in human mesothelial cells and in causing mesothelioma in hamsters. Proc Natl Acad Sci USA. 2006; 103:14128- 33.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "SV40 (MRQ-4) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "Le SV40 (MRQ-4) Mouse Monoclonal Primary Antibody est destiné à un usage en laboratoire pour détecter l’antigène du virus simien 40 (SV40) dans les tissus fixés au formol et enrobés à la paraffine, colorés sur les instruments VENTANA BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Product Purpose" } ] } } ] }