For in vitro diagnostic use. Others cobas DNA Sample Preparation Kit IVD cobas® DNA Sample Preparation Kit RMD-4800-014 05985536190 KIT DNA ISOLATION 24T IVD cobas DNA Sample Preparation Kit 00875197004120 Reagents, kits 1 kit 24 tests false The cobas® DNA Sample Preparation Kit is used for manual sample preparation to isolate genomic DNA from formalin-fixed paraffin-embedded tumor tissue (FFPET) samples. en Sample preparation FFPET samples are processed and genomic DNA isolated using the cobas® DNA Sample Preparation Kit, a generic manual sample preparation based on nucleic acid binding to glass fibers. A deparaffinized 5-micron (μm) section of an FFPET sample is lysed by incubation at an elevated temperature with a protease and chaotropic lysis/binding buffer that releases nucleic acids and protects the released genomic DNA from DNases. Subsequently, isopropanol is added to the lysis mixture that is then centrifuged through a column with a glass fiber filter insert. During centrifugation, the genomic DNA is bound to the surface of the glass fiber filter. Unbound substances, such as salts, proteins and other impurities, are removed by centrifugation. The adsorbed nucleic acids are washed and then eluted with an aqueous solution.The amount of genomic DNA can be spectrophotometrically determined and adjusted to a fixed concentration. en