Electro-chemiluminescence immunoassay (ECLIA) for the in vitro quantitative determination of S100 (S100 A1B and S100 BB) in human serum
This assay can be used
ECL (ElectroChemiLuminescence) is Roche’s technology for immunoassay detection. Based on this technology and combined with well-designed, specific and sensitive immunoassays, Elecsys delivers reliable results. The development of ECL immunoassay is based on the use of a ruthenium-complex and tripropylamine (TPA).
The chemiluminescence reaction for the detection of the reaction complex is initiated by applying a voltage to the sample solution, resulting in a precisely controlled reaction. ECL technology can accommodate many immunoassay principles while providing excellent performance.
* LoB = Limit of Blank; LoD = Limit of Detection; LoQ = Limit of Quantitation (the lowest analyte concentration than can be reproducibly measured with an intermediate precision CV of ≤ 20 %)
**defined by the lower detection limit and the maximum of the master curve
Source: S100 Method Sheet 08817324500V5.0 and Elecsys S100 Method Sheet 03175243190V13.0