Multiplexed assays for simultaneously detecting several biomarkers in a single sample address the growing need to obtain more information from limited tissue samples
New test kit is an addition to line of DISCOVERY ULTRA instruments, uniquely designed for the research community
Researchers can now investigate more cancer biomarkers on a single tissue slide, paving the way for a better understanding of cancer biology and future advances in personalised healthcare
Tucson, AZ, 18 March 2021 – Roche (SIX: RO, ROG; OTCQX: RHHBY) today announced the launch of the DISCOVERY Green HRP kit,1 the newest addition to the comprehensive collection of modular-based detection kits to identify and profile biomarkers and cell populations in tissue-based research. Advances in the understanding of cancer biology can help pave the way for improved cancer testing for patients.
The DISCOVERY Green HRP (horseradish peroxidase) kit can be used in combination with other detection kits, expanding the multiplexing capability of both immunohistochemistry (IHC) and in situ hybridisation (ISH) by providing a distinct colour that contrasts with other chromogens. DISCOVERY Green HRP complements chromogens including DISCOVERY Yellow, DISCOVERY Purple, DISCOVERY Red and DISCOVERY Teal, providing a variety of chromogens to choose from for multiplexing up to seven biomarkers simultaneously.
As IHC multiplexing technology expands to more markers, researchers need chromogens that can provide greater contrast for image analysis,
said Jill German, Head of Roche Pathology Customer Segment.
DISCOVERY Green HRP was designed and developed with researchers in mind and will enable them to see multiple biomarkers instantly, with the ultimate goal of advancing patient care globally.
The innovative DISCOVERY chromogens have translucent properties that enable researchers to analyse the co-localisation of multiple biomarkers through the spatial overlapping of two signals in the context of tissue.
We use chromogens like DISCOVERY Green, which has good spectral separation from existing favorites,
said Dr. John Le Quesne, Pathologist at the University of Cambridge, UK.
They provide good translucency and better performance in simple image analysis than unmixing traditional chromogens.
Roche’s DISCOVERY Green HRP kit is supplied2 in ready-to-use dispensers and provides a stable staining signal during alcohol dehydration. It can be fully automated on the DISCOVERY ULTRA system.3