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Multiplate® analyzer

A reliable tool for testing platelet function1

Multiplate® analyzer
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The Multiplate® Analyzer can detect platelet dysfunction and thus may be used to aid in the diagnosis and clinical management of patients with platelet function disorders.2,3

Blood platelets play a pivotal role in physiological hemostasis. Disorders of platelet function in general manifest most clearly as either bleedings or thrombotic disorders, hence platelet function testing can be utilized in the analysis of inherited and acquired platelet function disorders. The Multiplate® Analyzer can detect platelet dysfunction and thus may be used to aid in the diagnosis and clinical management of patients with platelet function disorders.2,3

 

Monitoring the patient’s response

 

The Multiplate® Analyzer may also be used to monitor the patient’s response to antiplatelet drugs and guide a selection of antiplatelet therapies.4 The Multiplate® Analyzer was the device used in the first large randomised controlled trial that demonstrated the safety and efficacy of switching of drug regimen based on platelet function testing (PFT), TROPICAL ACS.5 In the TROPICAL-ACS study, patients with acute coronary syndrome undergoing PCI were treated with either 1) one week of prasugrel followed by 1 week of clopidogrel and PFT-guided maintenance therapy with clopidogrel or prasugrel or 2) with prasugrel only. There were no differences in ischaemic and bleeding events in both groups with this approach. The authors conclude: “Guided de-escalation of antiplatelet treatment was non-inferior to standard treatment with prasugrel at 1 year after PCI in terms of net clinical benefit”. Hence early de-escalation of antiplatelet treatment from prasugrel to clopidogrel guided by Multiplate® platelet function testing results could be an alternative approach to standard treatment regimens.5

 

Pre-surgical and/or perioperative tool

 

The Multiplate® Analyzer may also be used as a pre-surgical and/or perioperative tool to aid in the prediction of bleeding and for monitoring the efficacy of various types of prohemostatic therapies.6-9 Studies have shown that Multiplate® results, used in conjunction with clinical presentation and additional laboratory markers, may be used to help determine timing of surgery after stopping antiplatelet therapy.10, 11 Multiplate® results may also be used to assess platelet function perioperative and could be integrated into institution transfusion algorithm to reduce platelet transfusion rates and hence risks from potential complications of platelet transfusion.12 Reduced platelet transfusions may also help to reduce costs.

multiplate-reagents-chart
ADPtest

Assay for the quantitative in vitro determination of platelet function following stimulation of the platelet adenosine diphosphate (ADP) receptors. The ADPtest assay may be used to detect an inhibition of the P2Y12 receptor, as well as an inhibition or absence of the GpIIb/IIIa receptor

ASPItest

Assay for the quantitative in vitro determination of platelet function triggered by arachidonic acid. The ASPItest may be used to detect an inhibition of the platelet cyclooxygenase, as well as an inhibition or absence of the GpIIb/IIIa receptor

TRAPtest

Assay for the quantitative in vitro determination of platelet function triggered by TRAP-6. Thrombin receptor activating peptide-6 (TRAP-6) is a potent platelet activator and stimulates platelet aggregation via the thrombin receptor PAR-1. The TRAPtest may be used to detect platelet function triggered via the thrombin receptor without triggering fibrin formation

GpIIbIIIa antagonist reagent

Inhibitor of the platelet GpIIbIIIa receptor. Addition of the GpIIbIIIa reagent to the blood sample leads to strongly reduced aggregation in the TRAPtest

Liquid Control Set

Quality control for electrical signal in impedance aggregometry based on the analysis of an artificial liquid control material

Multiplate analyzer
Helping to guide cost-effective therapeutic approaches
  • in coronary interventions5,13,14
  • in cardiac surgeries10,15
  • in vascular surgeries9,15
  • in peripheral vascular interventional procedures16 
  • prevention of stent occlusion/reocclusion17 

 

Fast and easy assessment

 

  • of platelet function from small volumes of whole blood3,18

 

Predictivity

 

  • for tailored anti-platelet regimen3,10
  • for stratification of bleeding risk in surgical and interventional procedures3,8,10
  • aid in diagnosis of inherited PF disorders3,10

 

Consistent results

 

  • Standardized reagents and procedures
  • Computer-based system, operates with automatic pipette and system performance controls

References

 

  1. Karon, BS. et. al. (2014), Clinical Chemistry, 60:1524-1531
  2. Gresele, P. et al. (2015), Haemost., 13:314-322
  3. Harrison, P. et. al. (2013), Hematol Oncol Clin N Am, 27:411-441 
  4. Aradi, D. et. al. (2015), Throm Haemost, 113:221-230
  5. Sibbing, D. et. al. (2017), Lancet, Aug 27 [e-pub]
  6. Ranucci, M. et al. (2011), Ann Thorac Surg, 91(1):123-9
  7. Weber, C.F. et al. (2012), Anesthesiology, Sep, 117(3):531-47
  8. Rafiq, S. et al. (2016), J Card Surg, 31(9):565-71
  9. Ferraris, VA. etl al. (2012), Ann Thorac Surg, 94:1761-1781
  10. Kong, R. et al. (2014), Int Jnl Lab Hem, 37:143-147
  11. Straub, N. et al. (2013). Thromb Haemost, 111(2):290-299
  12. Pape, A. et. al (2010), ISBT Science Series, 5:161-168
  13. Aradi, D. et. al. (2014), Euro Heart J, 35:209-215
  14. Aradi, D. et. al. (2013), Int J Cardiol, 167:2140-2148
  15. Ranucci, M. et. al. (2017), Interact Cardiovasc Thorac Surg, 24:196-202
  16. Rajagopalan, S. et. al. (2007), J Vasc Surg, 46:485-490
  17. Byrne, R.A. et al. (2015), Euro Heart J, 36:3320-3331 
  18. Roche (2021) Multiplate® analyzer Operator's Manual version 5.0 Software version 2.06

 

Not for diagnostic use in the US.

 

Overview

Ordering Information

Technical Documents

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System specifications

  • High throughput

    30 tests/hour

  • Sample

    Whole blood sample anticoagulated with hirudin eliminates need for sample preparation, reduces risk of pre-analytical errors

  • Low sample volume

    only 300 μL whole blood per analysis

  • Fast turn-around time

    10 min./test

  • Easy to use

    only 3 pipetting steps

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