For in vitro diagnostic use. Cell Membrane Cytoplasm Others anti-c-KIT 9.7 CONFIRM Primary Antibody IVD Gastric Carcinoma Mast Cell-Containing Tissue GIST CONFIRM™ anti-c-KIT (9.7) Primary Antibody PID00000556 Mouse 9.7 05 277 981 001 5 277 981 001 05277981001 5277981001 05277981001 CONFIRM ANTI-C-KIT (9.7) PRIMARY ANTIBOD CONFIRM anti-c-KIT (9.7) Primary Antibody 04015630970049 Reagents, kits 790-2222 50 tests Not Available undefined This antibody is intended for in vitro diagnostic (IVD) use.Ventana Medical Systems' (Ventana) CONFIRM anti-c-KIT (9.7) Primary Antibody is a rabbit monoclonal antibody (IgG) directed against the C terminal (intracellular) domain of c- KIT protein and recognizes both wild type and mutant forms of the c-KIT protein. This antibody is intended for laboratory use, via light microscopy, for the qualitative detection of c-KIT protein in formalin-fixed, paraffin-embedded tissue on a Ventana automated slide stainer. CONFIRM anti-c-KIT (9.7) specifically binds to antigen located in the cell membrane and cytoplasmic regions of normal mast cells, interstitial cells of Cajal (ICC), and gastrointestinal stromal tumors (GISTs).This antibody does not cross react with PDGFR alpha, c-FMS or Flt-3. The presence of c-KIT protein in GISTs is helpful diagnostically in the identification of GISTs and their separation from other stromal lesions.1 The clinical interpretation of any staining, or the absence of staining, must be complemented by morphological studies and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests. Prescription only. 1. Sarlomo-Rikala M, Kovatich H, Barusevicius A, Miettinen M. CD117: A sensitive marker for gastrointestinal stromal tumors that is more specific than CD34. Mod Pathol (1998) 11: 728-734. en CONFIRM anti-c-KIT (9.7) may be used as the primary antibody for immunohistochemical staining of paraffin tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and cover slipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.4CONFIRM anti-c-KIT (9.7) is optimally diluted for use with Ventana detection kits and automated slide stainers. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the Ventana automated slide stainer to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen containing slide a coverslip solution is applied in the slide stainer. Staining is completed after incubation with a substrate chromogen and optional counterstaining. For more detailed information on instrumeinstrument operation, refer to the appropriate Ventana automated slide stainer Operator's Manual. 4. Taylor C (Clive Roy). Immunomicroscopy : a diagnostic tool for the surgical pathologist / Clive Roy Taylor; James L. Bennington, consulting editor. Major problems in pathology; v. 19 Philadelphia: Saunders, 1986. en

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CONFIRM^™ anti-c-KIT (9.7) Primary Antibody

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