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"StructureGroupName": "BenchMark", "StructureNodeStatus": "Active" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Store at 2 to 8°C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "CONFIRM anti-CD4 (SP35) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of CD4 by light microscopy in sections of formalin-fixed, paraffin- embedded tissue stained on a BenchMark IHC/ISH instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "CD4 is a 55 kDa monomeric type I transmembrane glycoprotein expressed by helper T- cells and at low levels in macrophages and dendritic cells but is absent on B-cells.1,2

CD4 acts as a co-receptor for the T-cell receptor and interacts directly with major histocompatibility complex class II molecules on antigen-presenting cells, which stimulate T-cell activation.1,2 CD4 is absent on immature T-cells and expressed in later stages of T-cell development as the cell matures into a helper T-cell.1-4 T-cell lymphomas derived from helper T-cells (e.g. adult T-cell leukemia, angioimmunoblastic T-cell lymphoma, mycosis fungoides) often retain CD4 expression while CD4 is generally absent in subtypes derived from cytotoxic T-cells or NK cells, such as enteropathy associated T-cell lymphoma, T-lymphoblastic lymphoma, and NK/T-cell lymphoma.1-4 Some T-cell lymphomas that exhibit variable CD4 expression include anaplastic large cell lymphoma and peripheral T-cell lymphoma.1-4

The detection of CD4 by immunohistochemistry (IHC) with the CONFIRM anti-CD4 (SP35) Rabbit Monoclonal Primary Antibody (CONFIRM anti-CD4 (SP35) antibody), when evaluated with other markers, may be used to aid in the identification of normal helper T-cells and sub-classification of T-cell lymphoma. The cellular staining pattern for CONFIRM anti-CD4 (SP35) antibody is membranous.

1. Dabbs DJ. Diagnostic Immunohistochemistry Theranostic and Genomic Applications, 5th edition. Vol 5. Amsterdam, Netherlands: Elsevier; 2019.
2. Naeim F. Principles of Immunophenotyping. In: Naeim F, Rao PN, Grody WW, eds. Hematopathology: Morphology, Immunophenotype, Cytogenetics, and Molecular Approaches. Cambridge, MA: Academic Press; 2009.
3. Swerdlow SH, Campo E, Harris NL, et al. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, 4th edition. Vol 4. Lyon, France: International Agency for Research on Cancer; 2008.
4. Higgins RA, Blankenship JE, Kinney MC. Application of immunohistochemistry in the diagnosis of non-Hodgkin and Hodgkin lymphoma. Arch Pathol Lab Med. 2008;132(3):441-461.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Content", "Value": "CONFIRM anti-CD4 (SP35) antibody contains sufficient reagent for 50 tests.

One 5 mL dispenser of CONFIRM anti-CD4 (SP35) antibody contains approximately 12.5 μg of a recombinant rabbit monoclonal antibody.
The antibody is diluted in Tris-HCl with carrier protein and 0.10% ProClin 300, a preservative. Specific antibody concentration is approximately 2.5 μg/mL.
There is no known non- specific antibody reactivity observed in this product.
CONFIRM anti-CD4 (SP35) antibody is a recombinant rabbit monoclonal antibody produced as purified cell culture supernatant.

Refer to the appropriate VENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, Material and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Staining, and Limitations.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "CONFIRM anti-CD4 (SP35) antibody binds to the CD4 protein in formalin-fixed, paraffin- embedded (FFPE) tissue sections. This antibody can be visualized using ultraView Universal DAB Detection Kit (Cat. No. 760-500 / 05269806001). Refer to the respective method sheet for further information", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "CONFIRM anti-CD4 (SP35) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of CD4 by light microscopy in sections of formalin-fixed, paraffin- embedded tissue stained on a BenchMark IHC/ISH instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }

CONFIRM anti-CD4 (SP35) Rabbit Monoclonal Primary Antibody

IVD For in vitro diagnostic use.
CONFIRM anti-CD4 (SP35) Rabbit Monoclonal Primary Antibody

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