For Research Use Only. Not for use in diagnostic procedures. Others LightMix Digital BRAF V600E RUO LightMix® Digital BRAF V600E 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000575 Kit with reagents for a maximum of 32 (HiSense plate) or 46 (Universal plate) digital PCR reactionsTIB MOLBIOL order number: 20-3002-32 10068540001 LightMix Digital BRAF V600E LightMix Digital BRAF V600E 04260159335187 Reagents, kits for a maximum of 32 (HiSense plate) or 46 (Universal plate) digital PCR reactions Not Available false The assay has been experimentally verified to a sensitivity of 0.05 % at 2 copies per partition (cpp) with an artificial target. With real samples the sensitivity may differ. en Using PCR methodology, a 117 bp fragment covering the BRAF V600E mutation is amplified with specific primers. The mutation site in the PCR fragment is analyzed using two labeled probes binding competitively to the mutation site. The mutant probe is FAM- labeled, and the wild type (WT) probe is HEX-labeled. The PC included in the kit contains 4-6 % synthetic mutant target in wild-type genomic DNA and is equivalent to ~17 ng/µl when dissolved in 60 µl of PCR grade water. It is recommended to always include at least one PC and a No-Template-Control (NTC) (e.g. PCR grade water) in each run. en