{ "ProductData": { "ID": "RTD001050", "ProductType": "Others", "BrandName": "Mucicarmine Staining Kit", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use.", "DisclaimerGroup1": "IVD", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "Formalin-Fixed Paraffin-Embedded Tissue (FFPET)" ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "Human tissue with epithelial mucin" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Mucicarmine Staining Kit" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Product_RTD001050_im_en.jpg", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "05-0025", "StructureNodeStatus": "Active", "NodeName": "Special Stains Staining Kits" }, { 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"ProductID": "INS_3198", "BrandName": "BenchMark Special Stains", "ProductNameAddition": "", "ReferenceType": "Instrument", "Classification": [ { "IdentifierofStructureSystem": "1", "NameofStructureSystem": "GPCH", "StructureNodeID": "7682", "StructureGroupPath": "Histology->Systems / Digital Products->Primary Staining->BenchMark Special Stains", "StructureGroupName": "BenchMark Special Stains", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "10-000-00", "StructureGroupPath": "Analyzer Instruments and Systems", "StructureGroupName": "Analyzer Instruments and Systems", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "080", "StructureGroupPath": "BenchMark", "StructureGroupName": "BenchMark", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "Lab_Type", 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"StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Mucicarmine Staining Kit should be stored at 2-8°C. Refrigerated kit components should be brought to room temperature prior to use.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "The Mucicarmine Staining Kit is intended for laboratory use as a qualitative histologic stain to demonstrate acid mucopolysaccharides (mucin) by light microscopy in sections of formalin-fixed, paraffin-embedded (FFPE) tissue stained on a BenchMark Special Stains instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This product is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Content", "Value": "The reagent vials are supplied in barcode labeled carriers to insert into the reagent tray of the instrument. Each kit contains sufficient reagent for 75 tests.

One 27 mL vial of Mucicarmine Stain reagent.
One 22 mL vial of Iron Hematoxylin A contains approximately 1% hematoxylin in 95% ethanol reagent.
One 27 mL vial of Iron Hematoxylin B contains approximately 1.2% ferric chloride and approximately 1% hydrochloric acid reagent.
One 22 mL vial of Tartrazine Counterstain contains approximately 1% tartrazine and approximately 1% acetic acid reagent.
Four vial inserts with sipping straws.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Background Information", "Value": "Mucicarmine staining is a modification of Southgate’s method. First described in 1896 by Mayer, mucicarmine staining is one of the oldest histochemical staining techniques for acid mucins; the most standardized version used in routine clinical practice is Southgate’s method.^1,2 Mucicarmine staining is a technique used to identify acid mucopolysaccharides, also called acid mucins, which are O-linked glycoproteins.^3,4 Mucicarmine stains carboxylated and sulfonated acid mucins; mucicarmine does not stain neutral mucins.²

Cryptococcus, a genus of pathogenic fungi, has an acid mucin rich capsule that is its dominant virulence factor.⁵ Detection of the acid mucin rich capsule of Cryptococcus using mucicarmine staining can be used to identify Cryptococcus.⁶

The Mucicarmine Staining Kit is used to demonstrate acid mucopolysaccharides (mucins) to aid the pathologist in the diagnosis of infection by Cryptococcus.


1. Dapson RW. The history, chemistry and modes of action of carmine and related dyes. Biotech Histochem. 2007;82(4-5):173-187.
2. Layton C, Bancroft, JD. Carbohydrates. In. Bancroft's Theory and Practice of Histological Techniques Eighth Edition: Elsevier; 2019:176-197.
3. Voet D, Voet, JG. Biochemistry 3rd Edition. 3rd ed: John Wiley & Sons; 2004.
4. Gurina T, Simms, L. Histology, Staining. In: Treasure Island, FL: Statpearls; 2020.
5. Casadevall A, Coelho C, Cordero RJB, et al. The capsule of Cryptococcus neoformans. Virulence. 2019;10(1):822-831.
6. Allison DB, Simner PJ, Ali SZ. Identification of infectious organisms in cytopathology: A review of ancillary diagnostic techniques. Cancer Cytopathol. 2018;126 Suppl 8:643-653.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "The staining reaction is based on the reaction of an aluminum-carmine chelate complex attached to acid groups of mucin.⁷ Mucicarmine contains an aluminum-carmine chelate complex that stains mucin pink to red. Iron Hematoxylin A and Iron Hematoxylin B stain the nuclei grey to black. Tartrazine Counterstain is applied to provide a contrasting yellow background.

This kit is optimized for use on BenchMark Special Stains instruments. The reagents are applied to tissue on microscope slides and mixed over the entire specimen.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "The Mucicarmine Staining Kit is intended for laboratory use as a qualitative histologic stain to demonstrate acid mucopolysaccharides (mucin) by light microscopy in sections of formalin-fixed, paraffin-embedded (FFPE) tissue stained on a BenchMark Special Stains instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This product is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }