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Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Cytoplasm Others Prostate Specific Antigen ER-PR8 IVD IVD Prostate Adenocarcinoma PSA (ER-PR8) Mouse Monoclonal Antibody RTD001100 IVD Mouse ER-PR8 05269334001 Cell Marque, PSA (ER-PR8) PSA (ER-PR8) 00841683100591 Reagents, kits 50 tests Not Available true This antibody is intended for in vitro diagnostic (IVD) use.The Cell Marque PSA (ER-PR8) antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. Use of this antibody is indicated, subsequent to clinical differential diagnosis, as an aid in the identification of prostatic adenocarcinoma within the context of an antibody panel, the patient’s clinical history, and other diagnostic tests evaluated by a qualified pathologist. en The stated primary antibody (760-4271) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffinembedded tissue sections. In general, immunohistochemical staining in conjunction with a streptavidin-biotin detection system allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. Alternatively, a biotin-free detection system may be used. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.The stated primary antibody (760-4271) is optimally diluted to be compatible with Ventana Roche detection kits and automated slide stainers. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the Ventana Roche automated slide stainer to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate Ventana Roche automated slide stainer Operator’s Manual. en