For in vitro diagnostic use. Others Red Counterstain II IVD Red Counterstain II RTD001108 05 272 017 001 5 272 017 001 05272017001 5272017001 05272017001 RED COUNTERSTAIN II Red Counterstain II 04015630972043 Reagents, kits 780-2218 100 tests Not Available true Red Counterstain II is intended to aid microscopic bright field observation for in situ hybridization applications. Red Counterstain II is for use with formalin-fixed, paraffin-embedded tissue on a BenchMark IHC/ISH instrument.This reagent is intended for in vitro diagnostic (IVD) use. en In general, in situ hybridization (ISH) staining allows the visualization of target DNA or RNA sequences via the sequential hybridization of a labeled DNA or RNA probe to the target, a primary antibody against the labeled probe, and a secondary antibody to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the target site. At the end of the incubation step, the instrument washes the slides to stop the reaction and remove unbound material.For ISH reactions that result in a visible blue precipitate, the slides may be counterstained with Red Counterstain II. Red Counterstain II contains a cellular dye, nuclear fast red, which forms chelates with aluminum cations, generating a pink color associated with cellular components.1 As a contrast, Red Counterstain II provides pink background staining in cell nuclei and cytoplasm of tissue sections to assist with the slide reading under bright field microscopy.In general, ISH staining allows the visualization of target DNA or RNA sequences via the sequential hybridization of a labeled DNA or RNA probe to the target, a primary antibody against the labeled probe, and a secondary antibody to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the target site. The specimen may then be counterstained and cover slipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes.1. Vacca LL, Laboratory Manual of Histochemistry, Raven Press, 1985. en

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