For in vitro diagnostic use. Others Silver ISH DNP Detection Kit VENTANA IVD VENTANA® Silver ISH DNP Detection Kit RTD001241 760-516 VENTANA Silver ISH DNP Detection Kit 07613336158418 60 tests true VENTANA SILVER ISH DNP DETECTION KIT 08318883001 Not Available Reagents, kits en The VENTANA Silver ISH DNP Detection Kit is an indirect system for detecting DNP- labeled targets. The kit is intended to identify targets by silver in situ hybridization (ISH) in sections of formalin-fixed, paraffin-embedded tissue that are stained on BenchMark IHC/ISH instruments.This product should be interpreted by a qualified reader in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in vitro diagnostic (IVD) use. en VENTANA Silver ISH DNP Detection Kit detects DNP-labeled probes bound to a specific sequence in formalin-fixed, paraffin-embedded (FFPE) tissue sections. The detection kit contains a primary antibody and an enzyme-labeled secondary antibody conjugated to horseradish peroxidase (HRP) which is used as the chromogenic enzyme. During the ISH staining process, DNP probes are co-hybridized to their respective specific target DNA sequences within the cell nuclei. VENTANA Silver ISH (SISH) DNP Detection Kit contains the following dispensers: mouse anti-DNP primary antibody labeled with hydroxyquinoxaline (HQ), mouse anti-HQ secondary antibody conjugated to horseradish peroxidase (HRP), Chromogen A (Silver A), Chromogen B (Silver B) and Chromogen C (Silver C). Following incubation with the HQ-labeled mouse anti-DNP primary antibody and then mouse anti-HQ HRP secondary antibody conjugate, the SISH reaction occurs. Briefly described, this reaction is driven by the sequential addition of Chromogens A (silver acetate), B (hydroquinone) and C (H2O2). Here, the silver ions (Ag+) are reduced by hydroquinone to metallic silver atoms (Ag0). This reaction is fueled by the substrate for HRP, hydrogen peroxide (Chromogen C). Figure 1 illustrates the SISH reaction. The specimen is then counterstained with Hematoxylin II for interpretation by light microscopy.The staining protocol consists of numerous steps in which reagents are incubated for predetermined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip which minimizes the evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with positive staining for the probe.For more detailed information on instrument operation, refer to the appropriate User Guide.