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"Name": "Storage Conditions (Product)",
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{
"Name": "Background Information",
"Value": "T-bet, a T-box transcription factor, is expressed in CD4+ T-lymphocytes committed to T-helper (T
h)1 T-cell development from naïve T-helper precursor cells (T
hp) and redirects T
h2 T-cells to T
h1 development. Anti-T-bet is a marker of mature T-cells and is expressed at very low levels in T
hp cells and is absent in precursor T-lymphoblastic leukemia/lymphoma cells. Scattered small lymphocytes in the interfollicular T-cell zone of reactive lymphoid tissue, including tonsil, lymph node, and spleen exhibited nuclear staining for anti-T-bet, with no anti-T-bet staining observed in germinal centers or mantle or marginal zones. T-bet is expressed in a significant subset of B-cell lymphoproliferative disorders, particularly at an early stage of B-cell development (precursor B-cell lymphoblastic leukemia/lymphoblastic lymphoma), and B-cell neoplasms derived from mature B-cells, including CLL/SLL, marginal zone lymphoma, and hairy cell leukemia. In contrast, B-cell neoplasms derived from pre-germinal center or germinal center B-cells, including mantle cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma are negative for T-bet.
1-71. Szabo SJ, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell. 2000; 100:665-69.
2. Zhang WX, et al. Cloning and characterization of a new member of the T-box gene family. Genomics. 2000; 70:41-8.
3. Jöhrens K, et al. T-bet transcription factor detection facilitates the diagnosis of minimal hairy cell leukemia infiltrates in bone marrow trephines. Am J Surg Pathol. 2007; 31:1181- 5.
4. Atayar C, et al. Expression of the T-cell transcription factors, GATA-3 and T-bet, in the neoplastic cells of Hodgkin lymphomas. Am J Pathol. 2005; 166:127-34.
5. Dorfman DM, et al. T-bet, a T-cell associated transcription factor, is expressed in a subset of B-cell lymphoproliferative disorders. Am J Clin Pathol. 2004; 122:292-7.
6. Harashima A, et al. Transcription factor expression in B-cell precursor-leukemia cell lines: preferential expression of T-bet. Leuk Res. 2005; 29:841-8.
7. Marafioti T, et al. Expression of B-cell lymphocyte-associated transcription factors in human T-cell neoplasms. Am J Pathol. 2003; 162:861-71.",
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