For in vitro diagnostic use. Others VENTANA EBER Probe IVD VENTANA® EBER Probe PID00000975 10 070 323 001 10070323001 VENTANA EBER Probe VENTANA EBER Probe 07613336227244 Reagents, kits 800-7239 30 tests Not Available true VENTANA EBER Probe is optimally formulated for use with VENTANA Magenta ISH DIG Detection Kit, ISH TSA Ancillary Kit, and accessory reagents on a BenchMark IHC/ISH instrument. The detection kit contains an HRP-labeled anti-hapten primary antibody, hapten-labeled tyramide amplification reagent, and magenta chromogen. During the ISH staining process, DIG-labeled probes are hybridized to their respective target sequences in the tissue. DIG-labeled EBER probe is detected with VENTANA Magenta ISH DIG Detection Kit. This detection system uses HRP-labeled anti-DIG mouse monoclonal antibody and DIG- labeled tyramide amplification to visualize the target as a magenta signal through the covalent linkage of sulforhodamine B-labeled tyramide to the tissue (see Figure 2). Figure 2. VENTANA Magenta ISH DIG Detection Refer to the method sheets of these detection reagents for additional information. The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip that minimizes the evaporation of the aqueous reagents from the slide. Results are visualized and interpreted using a light microscope. en VENTANA EBER Probe is intended for laboratory use in the qualitative in situ hybridization (ISH) detection of Epstein-Barr encoded RNA by light microscopy in sections of formalin-fixed, paraffin- embedded tissue stained on a BenchMark IHC/ISH instrument. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This product is intended for in vitro diagnostic (IVD) use. en

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