Digital LightCycler® System

Unleash the true power of digital PCR

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The 4 pillars of advancement*
Sensitivity

 

The Digital LightCycler® System has an enhanced ability to detect minimal DNA or RNA molecules:

  • High-sensitivity 20,000 partition plate w/ 45μl reaction volume
  • 5x concentrated master mixes (RNA, DNA) enable high sample utilization, which means more confidence in results
  • Little rain and clear cluster separation means setting thresholds is easy
Precision

 

The Digital LightCycler® System’s high resolution 100,000 plate offers laboratories:

  • The ability to more likely discriminate small differences between samples
  • Superior cluster tightness and clarity of results
  • Absolute quantification of results
Flexibility

 

The Digital LightCycler® System brings all these advancements together on one system, offering a bold step forward in digital PCR:

  • Choose batch size increments of between 8 and 96 samples per run
  • Combine lanes for results of a single sample to increase resolution
  • Simplify workflow with sample tracking and automated data analysis
  • Enable high multiplexing with a 6 optical channel analyzer (Demonstrated with both DNA and RNA reagents)
  • Address multiple challenges at once with three plates for different application needs (Universal, High Sensitivity, High Resolution)

Quick, clear, reproducible results give great confidence with the potential for shorter turnaround for publishable scientific research and the potential development of clinically viable assays.

Integration

 

Bringing it all together, the Digital LightCycler® System brings reliable confidence in results:

  • Chemistry of generic reagents:
    • 5x concentrated master mixes means greater potential sample volume input
    • DNA and RNA optimized for multiplexing
    • Filling dye control that validates partitioning
  • 6 optical channels
  • Nanowell plate types: Solid phase partitioning instead of droplets increases reliability and replicability
  • Closed system minimizes the chance of amplicon contamination
  • Laboratory Information Digital LightCycler® System connectivity means simple digital workflow and sample tracking
  • Algorithm for sophisticated rejection of non-PCR artifacts
  • Absolute quantification without the need for standard curves
Bespoke testing with 3 one-of-a-kind1-4 nanowell plates

The unique hexagonal-shaped nanowell partitions and the filling dye that validates partitioning gives the Digital LightCycler® System the ability to handle a wide variety of extracted samples such as FFPET, cfDNA, Whole Blood, and more.

Through these partitions, target sequences are efficiently concentrated within isolated microreactors. This concentration effect reduces template competition, thereby enabling the detection of rare mutations and allowing for a higher tolerance to inhibitors present in a sample.

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High Resolution

 

15μL, 100,000 partitions

Copy Number Variation

NIPT

Human Genetic Disease

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Universal Plate

 

30μL, 28,000 partitions

Gene Expression

Transplant Rejection

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High Sensitivity

 

45μL, 20,000 partitions

Cell-free DNA

Oncology

Rare Mutation Detection

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Increased flexibility with 6-channel multiplexing*

6 advanced optical channels enable a high degree of multiplexing for DNA or RNA targets.

 

  • Batch size increments of between 8 and 96 samples per run
  • Demonstrated with both DNA and RNA reagents
  • Analyzer run times vary for different batch sizes to save time on smaller batches
High sample utilization with with 5x concentrated concentrated DNA and RNA master mixes*

Get the most out of your samples with the Digital LightCycler® System's 5x concentrated master mixes.

 

  • Improved sample percentage in each reaction means confidence in results
  • 1 Step RNA (RT) with a hold time of just 15 minutes
  • DNA and RNA optimized for multiplexing
  • Filling dye control that validates partitioning
  • Single tube
  • 50-75% less primer/probe usage
digital lightcycler sample utilization vs competitive system

Simple workflow and data tracking

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1. First batches are created either manually or with orders from the Laboratory Information System (LIS). The system automatically sets up the layout and creates the samples.

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2. The newly extracted samples are then samples are then transferred to the PCR where a pipette is used to mix the samples and the master mix and the nanowell plate of choice is scanned into the web application for full sample tracking.

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3. The PCR mix is then transferred into the nanowell plate which is then loaded into the partitioning engine. This is done for each plate.

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4. All the plates are then loaded into the Analyzer.

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5. After the run is complete, positives and negatives are counted up and the Poisson calculation is applied.

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6. Results are validated and then sent back to the LIS.

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Focus on core tasks with comprehensive customer support

The Digital LightCycler® Service Software will help automate workflow and manage data ensuring no external calculations, software and processors will be required to collate, analyze, and contextualize assay results.

 

  • Remote accessible software
  • Automatic clustering
  • Flexible for development of assays
  • Build your own In-Vitro Diagnostics
  • Analysis types
    • Absolute Quantification
    • Indel
    • Copy Number Variation
  • Full sample tracking
  • Laboratory Information System interface
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Designed to make workflows simple and easy

Find out how the latest digital PCR systems are impacting modern research laboratories and opening up new avenues for discovery.

 

Download the workflow brochure

A comprehensive suite of powerful features

View Full Table

A comprehensive suite of powerful features

Backed up by a strong history of IVD leadership and recognition with the original LightCycler® System, the Digital LightCycler® has a combination of unique features that make it a powerful competitor in the digital PCR space.1-4

 

Competitor A

Competitor B

Competitor C

Competitor D

Roche

So what?

Partitions

20,000 8,000 - 26,000 30,000 20,000 20,000 - 28,000 - 100,000 More applications

Samples

1 - 96 24, 96 4,12 1-24 8-96 Equivalent efficiency

Concentration on MMx

4x  4x 2x 2x 5x More sample volume

Channels

5 5 6 2 6 Equivalent multiplexing

Total volume

20μL 15μL 25μL 20μL 15μL - 45μL More opportunities

MD Label

Yes No No No Yes Confidence

Unambiguous thresholding = Confident results

 

Backed up by a strong history of IVD leadership and recognition with the original LightCycler® System, the Digital LightCycler® has a combination of unique features that make it a powerful competitor in the digital PCR space1-4.

  • Clear cluster separation of positives and negatives
  • Very little rain

 

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Roche Digital LightCycler® System

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Competitor A

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Competitor B

A wide range of research possibilities

View Full Table

A wide range of research possibilities

The Digital LightCycler® System’s unique precision and sensitivity capabilities make it ideally suited for a number of real-world applications and research areas:

Application Oncology

Rare species and

variant detection

Microsatellite

instability detection

Pathogen load

Digital

LightCycler® 

System Potential

Known specific variants of interest and tracking of treatment efficacy and disease progression  Absolute quantification without a standard curve Hyper sensitivity Absolute quantification with a standard curve
Application Graft rejection monitoring in transplant cases

ID - Environmental

surveillance

Pharma - Large

clinical trials

Noninvasive

prenatal testing

Digital

LightCycler® 

System Potential

Ability to measure graft cfDNA (est. Molecular Market 600 mio CHF)

High sensitivity and precision.

Inhibitors for particular sample

types may mask NA content

making difficult the rtPCR

curve analysis

Large volume testing for specific

biomarkers & larger accuracy

than rtPCR

Limited accuracy with antigen

testing

Registration status

IVD

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Making the leap from idea to impact

Modern clinical research demands a leap forward in instrumentation capability. With its flexibility, quality and advances in sensitivity and precision, the Digital LightCycler® System can help laboratories push the boundaries of clinical research forward

 

Unleash the true power of digital PCR with the Roche Digital LightCycler® System.

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Explore the oligo houses of our Partner Millipore Sigma

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* Roche data on file: DH_02365.01_031B_Digital_LightCycler_Reagent_Feasibility_Report_v3, Document Number: 0000000000001200000501942

Roche data on file: DH-02365.01-500E_Digital LightCycler System Performance report

Roche data on file: DHF Digital_LightCycler_Reagent_Feasibility_Report

Roche data on file: DH-02365.01-008B_Digital_LightCycler_System_Feasibility_Report

  1. https://www.qiagen.com/us/products/instruments-and-automation/pcr-instruments/qiacuity-digital-pcr-system/ (last accessed on 06/17/2022)
  2. https://www.bio-rad.com/en-us/life-science/digital-pcr/qx-one-droplet-digital-pcr-ddpcr-system (last accessed on 06/17/2022)
  3. https://www.stillatechnologies.com/6-color-dpcr/ (last accessed on 06/17/2022)
  4. https://www.thermofisher.com/order/catalog/product/4489084 (last accessed on 06/17/2022)