See country-specific product labeling for regulatory status. Instrument LightCycler 2.0 Country Specific Labeling LightCycler® 2.0 Instrument INS_409 03531414001 LightCycler 2.0 LightCycler 2.0 08430215046203 Instruments 1 instrument Not Available plus related products and data station The LightCycler® 2.0 Instrument enables the monitoring of amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately.Amplification occurs in specially designed reaction capillaries. Their high surface-to-volume ratio provides very rapid thermal transfer, which facilitates high-speed thermal cycling.The combination of LightCycler® Capillaries and the air-driven temperature control of the LightCycler® 2.0 Instrument ensures rapid PCR. An entire 35-cycle run can be performed in as little as 30 minutes (with 20 μl capillaries) to 60 minutes (with 100 μl capillaries).Fluorescence detection formats: The LightCycler® 2.0 Instrument is optimized for three fluorescence detection formats: SYBR Green I, HybProbe probes and hydrolysis probes. In addition, the instrument supports a wide variety of other fluorescence detection formats, such as monocolor SimpleProbe probes and other formats based on FRET (fluorescence resonance energy transfer).Software: The LightCycler® 2.0 Instrument utilizes the LightCycler® Software 4.1, an optimized version of the LightCycler® 4.0 and 4.05 Software, which provides advanced data analysis modules, efficient data management and effective data protection. en Use the LightCycler® 2.0 Instrument to perform:Fast and sensitive real-time PCR and RT-PCRReproducible and reliable kinetic quantification of DNA, cDNA, or RNAEasy PCR product identification and characterization by melting point analysisHighly specific mutation analysis and SNP (Single Nucleotide Polymorphism) genotyping en The LightCycler® 2.0 Instrument is intended for performing rapid PCR (Polymerase Chain Reaction) with real-time detection and/or quantification of a target NA (nucleic acid), as well as post-PCR analysis of the amplified NA by melting curve analysis. The LightCycler® 2.0 Instrument is designed for in vitro diagnostic applications in combination with the LightCycler® reagent kits manufactured by Roche and labeled for diagnostic purposes (according to the workflow described in the package insert of the respective LightCycler® reagent kit).Please note: The Roche LightCycler® reagent kits for in vitro diagnostic applications requiring this instrument are not available in all countries. Any diagnostic use of the LightCycler® 2.0 Instrument in combination with the LightCycler® reagents (other than those labeled for diagnostic purposes, manufactured by Roche and recommending the LightCycler® 2.0 Instrument in their instructions of use) is in the sole responsibility of the user and has to be validated by the user, taking into account all relevant national legislation.Customers using Roche in vitro diagnostic LightCycler® kits have to use “Manual A, marked for in vitro diagnostic use” (not available in all countries; id. no. 05 002 702 001; to obtain your local language version contact your Roche representative).The LightCycler® 2.0 Instrument must be used exclusively by laboratory professionals trained in laboratory techniques and having studied the instructions for use of this instrument. en