For in vitro diagnostic use. Others ISH iView Blue Detection Kit IVD VENTANA® ISH iVIEWBlue Detection Kit RTD000977 05278511001 ISH IVIEW BLUE DETECTION KIT ISH iVIEW Blue Detection Kit 04015630971930 Reagents, kits 800-092 200 tests Not Available true VENTANA ISH iVIEW Blue Detection Kit ist ein indirektes Biotin-Streptavidin-System für den Nachweis von fluoresceinmarkierten Sonden. Das Kit ist für die Identifizierung von Zielsequenzen durch In-situ-Hybridisierung in Schnitten von formalinfixiertem, paraffineingebettetem Gewebe, die auf einem BenchMark IHC/ISH Gerät gefärbt wurden, bestimmt. Dieses Produkt muss von einem qualifizierten Pathologen in Verbindung mit histologischen Untersuchungen, klinisch relevanten Informationen und geeigneten Kontrollen interpretiert werden.Dieses Produkt ist für die Verwendung in der In-vitro-Diagnostik (IVD) bestimmt. de VENTANA ISH iVIEW Blue Detection Kit detects specific fluorescein-labeled probes bound to specific target sequences in formalin-fixed, paraffin-embedded (FFPE) tissue sections. VENTANA ISH iVIEW Blue Detection Kit contains mouse anti-fluorescein primary antibody that detects the fluorescein-labeled probes bound to the target sequence. The anti-fluorescein antibody is followed by the binding of a biotinylated secondary antibody consisting of goat anti mouse IgG. This step is followed by the addition of a Streptavidin-AP (alkaline phosphatase) enzyme conjugate which binds to the biotin present on the secondary antibody. The complex is then visualized with 5-Bromo-4- chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) chromogen, which produces a blue precipitate that is readily detected by light microscopy.Unlike horseradish peroxidase, alkaline phosphatase is unaffected by endogenous peroxidase activity and is therefore more effective for labeling specimens heavily infiltrated with neutrophils or eosinophils. Nitro blue tetrazolium stains the tissue blue and has the advantages of a higher extinction coefficient, yielding a more intense color reaction at the site of probe target interaction.The staining protocol consists of numerous steps in which reagents are incubated for predetermined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip which minimizes the evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with positive staining for the probe. en