For in vitro diagnostic use. Others VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail IVD VENTANA® Kappa and Lambda Dual ISH mRNA Probe Cocktail PID00000773 CE-IVD 08 507 023 001 8 507 023 001 08507023001 8507023001 08507023001 VENTANA K L DISH mRNA PRB CKT-USExport VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail 07613336160558 Reagents, kits 800-6054 30 tests Not Available true VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail is intended for the qualitative detection of Kappa mRNA and Lambda mRNA in formalin-fixed, paraffin-embedded (FFPE) human bone marrow and lymphoid tissue stained on a BenchMark IHC/ISH instrument using chromogenic in situ hybridization (ISH) and visualized using light microscopy. VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail is intended as an aid in the identification of B-cell lymphomas and plasma cell neoplasms.Results of the assay should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in vitro diagnostic (IVD) use. en VENTANA K/L Probe Cocktail is formulated for use with the VENTANA Magenta ISH DIG Detection Kit, the VENTANA Silver ISH BF Detection Kit, the ISH TSA Ancillary Kit, and accessory reagents on a BenchMark IHC/ISH instrument.The detection kits contain HRP-labeled anti-hapten primary antibody, hapten-labeled tyramide amplification reagent, and silver or magenta chromogen. During the ISH staining process, BF and DIG-labeled probes are hybridized to their respective target sequences in the tissue.DIG-labeled kappa probe is detected with VENTANA Magenta ISH DIG Detection Kit. This detection system uses HRP-labeled anti-DIG mouse monoclonal antibody and DIG- labeled tyramide amplification to visualize the target as a magenta signal through the covalent linkage of sulforhodamine B labeled tyramide to the tissue (see Figure 2).Figure 2. VENTANA Magenta ISH DIG Detection Kit BF-labeled lambda probe is detected with VENTANA Silver ISH BF Detection Kit. This detection system uses HRP-labeled anti-BF mouse monoclonal antibody and BF-labeled tyramide amplification to visualize the target as a black signal through the precipitation of silver (see Figure 3).Figure 3. VENTANA Silver ISH BF Detection Kit Refer to the method sheets of these detection reagents for additional information.The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark instrument washes the sections to remove unbound material and applies a liquid coverslip to minimize evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope.For more detailed information on instrument operation, refer to the appropriate User Guide. en

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VENTANA^® Kappa and Lambda Dual ISH mRNA Probe Cocktail

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