Article

AXELIOS 1 data analysis

Roche Diagnostics
Published on June 29, 2026 | 2 min read
A person sits facing a laptop with cloud and data icons, representing AXELIOS 1 data analysis capabilities.

Enabling the future of NGS with SBX-optimised integrated and standalone analysis tools

The AXELIOS 1 Platform is set to shape the future of NGS with the speed, accuracy, scalability and flexibility it offers. With the advent of this novel technology comes the need for analysis tools equipped to maximise its value. To do this, the platform combines the speed and flexibility of integrated analysis workflows with the customisability and performance of standalone XOOS analysis tools. This comprehensive approach delivers the acceleration and versatility needed to transform high-throughput sequencing data into biological insights.

Integrated analysis

AXELIOS 1 integrated analysis workflows accelerate the path to discovery by analyzing data where it’s generated, as it’s generated. These workflows combine high-performance compute with real-time sequencing data processing, spanning base calling to mapped and aligned reads* while the run is underway.1 As a result, the AXELIOS 1 Platform offers exceptionally rapid turnaround times for multiple data outputs in standard file formats.**

Flexible data off-ramps for diverse research needs

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Flexible data off-ramps for diverse research needs

AXELIOS 1 Platform offers multiple data off-ramps within the workflow to provide maximum flexibility in meeting a diverse set of sequencing workflow and application development needs.

AXELIOS 1 Platform offers multiple data off-ramps within the workflow to provide maximum flexibility in meeting a diverse set of sequencing workflow and application development needs.

Data output type Supported SBX-workflows Output format Characteristics
Raw reads SBX-D, SBX-S fastq.gz (non-demultiplexed)
  • Provides access to furthest upstream point of SBX data, directly resulting from the base calling process
  • Enables highest degree of flexibility and customisation of user-driven SBX application and analysis development
Consensus reads SBX-D fastq.gz (sample-specific)
  • Provides access to high quality (>Q38) reads to support several applications and detection of multiple variant types
  • Enables significant flexibility and customisation of user-driven SBX application and analysis development with high quality data
Mapped/aligned reads SBX-D BAM (sample-specific, indexed)
  • Optimised, efficient data flow from raw sequencing data to mapped and aligned reads
  • Maximises value of SBX read generation rates and modality
  • Reduced data footprint
  • Directly used for downstream secondary analysis

Standalone XOOS analysis tools

The speed, flexibility, and efficiency of AXELIOS 1 Platform integrated compute is complemented by our suite of customisable, SBX-optimised, XOOS (pronounced “zoose”) analysis tools. Hosted on Github and capable of being deployed on local or cloud compute infrastructure, these high-performing, scalable tools provide foundational data analysis building blocks for creating robust data pipelines and enabling the SBX analysis ecosystem for a diverse range of applications and variant types.

Access the data set

Create your account to access the whole genome sequencing SBX-D genome in a bottle (GIAB) data set.

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AXELIOS 1 data analysis resources

XOOS repository hosted by Roche on GitHub

XOOS analysis tools

XOOS analysis tools are hosted by Roche on Github

Visit XOOS repository

XOOS documentation hosted by Roche on GitBook

Analysis tool documentation

Supporting documentation for XOOS analysis tools is hosted by Roche on Gitbook

Visit XOOS documentation

Github Deep Variant logo

DeepVariant model trained for SBX

Github repository for DeepVariant

Visit DeepVariant

Screenshot of a Roche white paper titled Germline variant calling using Sequencing by Expansion with DeepVariant.

Download our white paper

Germline variant calling using SBX with DeepVariant

Explore how SBX, when coupled with advanced bioinformatics tools like a pangenome aligner and DeepVariant, provides accurate germline variant identification from whole-genome sequencing (WGS) data.

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SBX-duplex data analysis webinar

SBX-duplex data and whole-genome germline small variant calling, supporting the release of a publicly available WGS SBX-D dataset.

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Contact us

Do you have questions about our sequencing products or services? We’re here to help. Contact a Roche representative today.

For Research Use Only. Not for use in diagnostic procedures. AXELIOS is a trademark of Roche.

*Real-time refers to the ability to process data from an ongoing sequencing run in parallel with said sequencing run.
Lag time between sequencing run completion and analysis completion is expected for both fastq.gz and bam outputs.
Ability to perform demultiplexing, intramolecular consensus and or mapping/alignment in real-time currently pertains to only SBX-D workflows.
Conversion to fastq.gz occurs in real-time.

**Analysis turnaround times may vary based on sequencing time, data output, data format, network bandwidth, analysis configurations and other experimental factors.

References

  1. Roche Diagnostics & GenomeWeb. Germline Small Variant Calling Workflow for SBX Duplex Data [Internet; cited 2026 Jun 15].  Available from: https://diagnostics.roche.com/global/en/events/sbx-d-data-analysis-webinar.html.
  2. Zook J, et al. Extensive sequencing of seven human genomes to characterise benchmark reference materials. Sci Data. 2015;3:160025.