For Research Use Only. Not for use in diagnostic procedures. Others KAPA EvoAmp RM Auto Kit RUO KAPA EvoAmp RM Auto Kit PID00001238 10 528 095 001 10528095001 KAPA EvoAmp RM Auto Kit KAPA EvoAmp RM Auto Kit 07613336237427 Reagents, kits 1 kit Not Available true The KAPA EvoAmp ReadyMix Kits are ideally suited for high-efficiency, high fidelity, low-bias amplification of libraries prior to Illumina sequencing. This includes libraries prepared for Whole-genome sequencing (WGS) and Whole exome sequencing (WES) or targeted sequencing. KAPA EvoAmp ReadyMix Kits which are powered by KAPA HiFi build on the existing performance of KAPA HiFi which is ideally suited for high-efficiency, high fidelity, low-bias amplification of libraries prior to Illumina sequencing. This includes libraries prepared for: whole-genome shotgun sequencing exome or targeted sequencing (pre- and post-capture amplification) RNA-seq ChIP-seq other sequencing applications In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. Amplification bias occurs when a DNA polymerase is unable to amplify all targets within a complex population of library DNA with equal efficiency. KAPA HiFi DNA Polymerase is a B-family DNA polymerase engineered for increased processivity, extremely high fidelity and low-bias, and is the reagent of choice for NGS library amplification.1,2,3,4 KAPA HiFi HotStart DNA Polymerase has 5’→ 3’ polymerase and 3’→ 5’ exonuclease (proofreading) activities. The error rate of KAPA HiFi HotStart DNA Polymerase is 2.8 x 10-7 errors/ base, equivalent to 1 error per 3.5 x 106 nucleotides incorporated. The enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This prevents nonspecific amplification during reaction setup, increases sensitivity, and improves reaction efficiency. KAPA EvoAmp ReadyMix, a ready-to-use Library Amplification mix comprising all the components for library amplification; except primers and template. 1. Oyola, S.O., et al., BMC Genomics 13, 1 (2012). 2. Quail, M.A., et al., Nature Methods 9, 10 (2012). 3. Quail, M.A., et al., BMC Genomics 13, 341 (2012). 4. Ross, M.G., et al., Genome Biology 14, R51 (2013). en

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