For Research Use Only. Not for use in diagnostic procedures. Others KAPA HiFi HotStart Library Amplification Kits RUO KAPA HiFi HotStart Library Amplification Kit SEQ-KAPA-0032 KAPA HiFi HotStart Library Amplification Kits are designed for the amplification of next- generation sequencing (NGS) libraries prepared for Illumina sequencing. In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. Amplification bias occurs when a DNA polymerase is unable to amplify all targets within a complex population of library DNA with equal efficiency. KAPA HiFi DNA Polymerase is a B-family DNA polymerase engineered for increased processivity, extremely high fidelity and low-bias, and is the reagent of choice for NGS library amplification.1,2,3,4 KAPA HiFi HotStart has 5'→3' polymerase and 3'→5' exonuclease (proofreading) activities. The error rate of KAPA HiFi HotStart DNA Polymerase is 2.8 x 10-7 errors/base, equivalent to 1 error per 3.5 x 106 nucleotides incorporated. The enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This prevents nonspecific amplification during reaction setup, increases sensitivity, and improves reaction efficiency. KAPA HiFi HotStart Library Amplification Kits include KAPA HiFi HotStart ReadyMix (2X), a ready-to-use PCR mix comprising all the components for library amplification; except primers and template. Kits also include the KAPA Library Amplification Primer Mix (10X), designed for the high-efficiency amplification of Illumina libraries flanked by adapters containing the P5 and P7 flow cell sequences. 1. Oyola, S.O., et al., BMC Genomics 13 , 1 (2012). 2. Quail, M.A., et al., Nature Methods 9 , 10 (2012). 3. Quail, M.A., et al., BMC Genomics 13 , 341 (2012). 4. Ross, M.G., et al., Genome Biology 14, R51 (2013). en KAPA HiFi HotStart Library Amplification Kits for Illumina platforms are ideally suited for high-efficiency, high-fidelity, low-bias amplification of libraries prior to Illumina sequencing. This includes libraries prepared for:whole-genome shotgun sequencingexome or targeted sequencing (pre- and post-capture amplification)RNA-seqChIP-seqother sequencing applicationsKAPA HiFi DNA Polymerase may also be used for other high-fidelity PCR applications. Please visit sequencing.roche.com to select the most appropriate KAPA HiFi formulation for your application.KAPA HiFi DNA Polymerase is not suitable for the amplification of templates containing uracil, such as bisulfite-treated DNA. For such templates, KAPA HiFi HotStart Uracil+ ReadyMix PCR Kits should be used. en

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