For in vitro diagnostic use. Others Hematoxylin II IVD Hematoxylin II RTD000939 05277965001 Hematoxylin II Hematoxylin II 04015630972135 Reagents, kits 790-2208 250 tests Not Available true Hematoxylin II is a modified Mayer's hematoxylin intended for laboratory use in staining cellular nuclei on slides containing cells from frozen tissue, or formalin fixed, paraffin‑embedded tissue on a BenchMark IHC/ISH instrument. This reagent is intended as a counterstain to immunohistochemistry, and in situ hybridization applications.This reagent is intended for in vitro diagnostic use. en In general, immunohistochemistry (IHC) allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site.Similarly, in situ hybridization (ISH) staining allows the visualization of target DNA or RNA sequences via the sequential hybridization of a labeled DNA or RNA probe to the target, a primary antibody against the labeled probe, and a secondary antibody to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the target site.For both applications, at the end of incubation step, the instrument washes the slides to stop the reaction and remove unbound material. The specimen may then be counterstained with Hematoxylin II solution which is applied to the slide and mixed over the entire specimen area. Hematoxylin II stains nuclei blue through the binding of a mordant dye complex to nucleic acids and histone proteins of the heterochromatin. Coverslip may then be applied to the specimen. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen or nucleic acid target. en