Next-generation sequencing (NGS) library preparation solutions

Prepare high-quality libraries for efficient next-generation sequencing.

Find more products Contact us
A lab technician carefully uses a pipette to add reagents from an NGS library preparation for sequencing kit to a vial

High-performing, comprehensive and streamlined NGS library preparation kits

Solution

NGS library prep kits for optimal performance, high yields, and uniform sequence coverage

Build better NGS libraries with KAPA library preparation kits, designed for unmatched performance on Illumina sequencing platforms.

  • Succeed with difficult samples: Overcome PCR inhibitors and get higher yields with our advanced, inhibitor-resistant solutions.
  • Trust your results: Minimize bias and achieve more uniform sequence coverage for data that accurately reflects your sample's biology.
  • Save time and reduce errors: Get consistent results with simplified, user-friendly workflows– including automated library prep– that feature ready-to-use master mixes that reduce pipetting steps.
  • Publish with confidence: Generate the high-quality, reliable data you need to draw confident conclusions in your research.

The connection between NGS library prep quality and sequencing

The NGS workflow is a multi-step process consisting of sample preparation, sequencing, and data analysis.

Library construction is a critical step in sample prep, which can also include other optional steps such as target enrichment. First, the DNA or RNA is fragmented to the appropriate size using various combinations of sound waves, enzymes, salts, and heat. For DNA samples, NGS adapters are then added (library conversion); a high conversion rate is one of the hallmarks of robust NGS library prep. Several types of adapters are available, but all include sequences that enable sequencing on a specific type of instrument, and most contain indexes that allow the final data to be associated with the correct sample. For RNA samples, the RNA is converted to cDNA prior to adapter ligation.

At each step, there is an opportunity for error and bias to arise– and this can negatively impact the quality and reliability of the results. High-quality reagents and optimized protocols can reduce these errors, and enable you to preserve sample integrity and reduce sample loss and bias while ensuring greater accuracy. In addition, the use of automated library prep not only reduces hands-on work but also increases the reproducibility of the data and reduces human error.

Roche offers comprehensive, streamlined KAPA library preparation kits for DNA and RNA; these high-performance kits yield libraries that are specifically designed for use on Illumina sequencing platforms. All of the kits in the KAPA NGS library prep portfolio contain high-quality enzymes selected through Directed Evolution Technology, an advanced engineering process that creates enzymes that are not only highly efficient but also exceptionally tolerant of common PCR inhibitors and less prone to bias.

The components are conveniently formulated in user-friendly master mixes, which are, in some cases, available in automation-ready plates. This simplifies NGS library preparation while delivering optimal performance, higher yields, and more uniform sequence coverage–ultimately leading to more reliable data and confident conclusions.

Value

Unlock deeper insights with NGS library prep solutions you can trust

Go from sample to high-quality sequencing data with confidence and efficiency. Roche’s integrated NGS library preparation solutions are designed to simplify your workflow, minimize errors, and deliver the reliable results your research demands.

With our complete and proven solutions, you can:

  • Maximize your efficiency: Spend less time on manual prep and more on discovery with automated library prep workflows.
  • Achieve uncompromising data quality: Generate high-fidelity, reproducible results by minimizing bias and errors with our integrated system of reagents and protocols.
  • Increase confidence in your results: Rely on rigorously validated solutions trusted by researchers worldwide to produce data that accurately reflects your sample's biology.
  • Simplify your entire workflow: Eliminate guesswork and complex steps with a complete solution designed for seamless performance from start to finish.
Vision

Advancing genomics, together

We believe the next great genomic discovery is locked within your samples, and our vision is to provide you with the key. We are relentlessly focused on advancing NGS library preparation to make it more precise, efficient, and reliable. We’re here to help you overcome technical hurdles and achieve superior sequencing outcomes, so you can spend less time on process and more time making the breakthroughs that matter.

Featured products

Streamlined, high-quality DNA library preparation kits for reliable sequencing results.

Explore DNA library preparation
Find more products

Streamlined, high-quality RNA library preparation kits for reliable sequencing results.

Explore RNA library preparation
Maximize Every Read. Our new KAPA RNA EvoPrep Kit shortens workflow, boosts sequencing efficiency, captures more unique transcripts, and accelerates discovery.
The KAPA RNA EvoPrep Kit with RiboErase (HMR or Human/Mouse/Rat) Globin contains all of the buffers and enzymes required for depletion of ribosomal RNA (rRNA) and globin mRNA transcripts, and the rapid construction of stranded RNA-seq libraries from 5 ng – 1 μg of blood-derived, purified total RNA. The KAPA RNA EvoPrep Kit with RiboErase (HMR) Globin is designed for both manual and automated NGS library construction. The kit depletes both cytoplasmic (5S, 5.8S, 18S, and 28S), and mitochondrial (12S and 16S) rRNA species as well as globin mRNA transcripts. The protocol is applicable to a wide range of RNA-Seq applications, including: gene expression analysis; single nucleotide variation (SNV) discovery; splice junction and gene fusion identification; and characterization of both polyadenylated and non-polyadenylated RNAs, including noncoding and immature RNAs. This kit has not been tested with small RNAs < 100 bp in length.
The KAPA RNA EvoPrep Kit with RiboErase (HMR or Human/Mouse/Rat) contains all of the buffers and enzymes required for depletion of ribosomal RNA (rRNA) and the rapid construction of stranded RNA-Seq libraries from 5 ng – 1 μg of purified total RNA. The KAPA RNA EvoPrep Kit with RiboErase (HMR) is designed for both manual and automated NGS library construction. The kit depletes both cytoplasmic (5S, 5.8S, 18S, and 28S), and mitochondrial (12S and 16S) rRNA species.This workflow is compatible with both high- and low-quality (e.g., extracted from FFPE tissue) RNA. The protocol is applicable to a wide range of RNA-Seq applications, including: gene expression analysis; single nucleotide variation (SNV) discovery; splice junction and gene fusion identification; and characterization of both polyadenylated and non-polyadenylated RNAs, including noncoding and immature RNAs. This kit has not been tested with small RNAs < 100 bp in lenght.
Find more products

Accurate, low-bias NGS library amplification and quantification reagents for high sequencing data quality and yields.

Explore Library amplification and quantification
KAPA Library Amplification Kits are next-generation solutions for NGS library amplification, delivering uniformity across the GC-range, and high-fidelity results.
KAPA Library Quantification Kits are a complete solution for accurate, reliable and reproducible qPCR-based quantification of NGS libraries.
KAPA EvoAmp ReadyMix contains KAPA HiFi HotStart DNA Polymerase that is specifically designed to minimize amplification bias, while maintaining extremely high fidelity. The KAPA EvoAmp ReadyMix is designed for efficient library amplification of adapter-ligated libraries for next-generation sequencing (NGS). KAPA EvoAmp ReadyMix is compatible with libraries constructed from input DNA amounts of 0.1 ng to 500 ng (high quality DNA). It is compatible with high-quality gDNA, cfDNA and low-quality DNA such as that extracted from formalin-fixed, paraffinembedded tissue (FFPET) samples. The KAPA EvoAmp ReadyMix Kits are designed for the amplification of next-generation sequencing (NGS) libraries prepared for Illumina sequencing. The KAPA EvoAmp ReadyMix Kits are ideally suited for high-efficiency, high fidelity, low-bias amplification of libraries prior to Illumina sequencing.This includes libraries prepared for Whole-genome sequencing (WGS) and Whole exome sequencing (WES) or targeted sequencing. KAPA EvoAmp ReadyMix Kits which are powered by KAPA HiFi HotStart build on the existing performance of KAPA HiFi which is ideally suited for high-efficiency, high fidelity, low-bias amplification of libraries prior to Illumina sequencing. This includes libraries prepared for: whole-genome shotgun sequencing exome or targeted sequencing (pre- and post-capture amplification) RNA-seq ChIP-seq other sequencing applications In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. Amplification bias occurs when a DNA polymerase is unable to amplify all targets within a complex population of library DNA with equal efficiency. KAPA HiFi DNA Polymerase is a B-family DNA polymerase engineered for increased processivity, extremely high fidelity and low-bias, and is the reagent of choice for NGS library amplification.1,2,3,4 KAPA HiFi HotStart DNA Polymerase has 5’→3’ polymerase and 3’→5’ exonuclease (proofreading) activities. The error rate of KAPA HiFi HotStart DNA Polymerase is 2.8 x 10-7 errors/ base, equivalent to 1 error per 3.5 x 106 nucleotides incorporated. The enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This prevents nonspecific amplification during reaction setup, increases sensitivity, and improves reaction efficiency. KAPA EvoAmp ReadyMix, a ready-to-use Library Amplification mix comprising all the components for library amplification; except primers and template. 1. Oyola, S.O., et al., BMC Genomics 13, 1 (2012). 2. Quail, M.A., et al., Nature Methods 9, 10 (2012). 3. Quail, M.A., et al., BMC Genomics 13, 341 (2012). 4. Ross, M.G., et al., Genome Biology 14, R51 (2013).
Find more products

NGS accessories, including validated adapters and cleanup beads, for high-quality library construction.

Explore NGS sample prep accessories
Find more products

Benefits of NGS library preparation solutions from Roche

Automation-enabled

Increase your throughput, not your workload

Streamline your NGS library prep from start to finish with our simplified, automation-friendly solutions. By combining ready-to-use reagents with optimized protocols, you can significantly reduce manual steps, improve consistency, and get to your results sooner.

  • For your current liquid handlers: Our automation team provides validated methods on many models for our library prep and target enrichment solutions.
  • For true walk-away freedom: The AVENIO Edge System automates the entire process.
Proven

Proven performance backed by over a decade of trust

Roche’s high-quality products, manufactured under stringent quality control to ensure lot-to-lot consistency, have served the research and pre-clinical research community for over a decade. They have been referenced in several thousand peer-reviewed research publications, demonstrating widespread validation and trust from scientists across the globe in numerous applications.

Among these trusted solutions, our KAPA Evo portfolio of NGS library preparation solutions provides exceptionally consistent performance. This reliability offers researchers the profound security of reproducible results and the workflow efficiency needed to accelerate discovery and achieve more with every sequencing run.

Complete

From precious sample to powerful insight

Our mission is to ensure that all of the biological information in each sample is preserved for analysis. Roche’s fully integrated system of premium reagents, consumables, and optimized protocols works together across the entire sample prep process to protect your samples from degradation and minimize artifacts.

This means you can move forward with confidence, knowing you are capturing the maximum amount of data for more accurate, meaningful, and publication-ready results.

Our solutions cover steps from nucleic acid extraction through library prep, target enrichment, and library QC:

  • KAPA NGS DNA Extraction kit, used to extract PCR-ready DNA from FFPE samples in the KAPA HyperPETE Somatic Tissue DNA Workflow

  • KAPA NGS FFPE DNA QC Kits for the qualification of sequenceable input FFPE DNA and calculation of the required input amount

  • KAPA EvoPrep Boost, KAPA EvoPlus Boost, and KAPA RNA EvoPrep Kits for flexible, single­ tube, high-efficiency library construction

  • KAPA EvoAmp ReadyMix for high-fidelity library amplification, plus KAPA HiFi Uracil+ for low-biased amplification

  • KAPA HyperCap Workflow with target enrichment hybridization-based products KAPA HyperExome V2, KAPA HyperChoice, and KAPA HyperExplore Probes for streamlined, versatile targeted sequencing

  • KAPA HyperPETE Workflow with KAPA HyperPETE Panels based on an innovative primer extension technology, enabling single-day high-performing target enrichment

  • KAPA Library Quantification Kits for accurate and reliable qPCR-based library quantification for multiplexed sequencing

You might be interested in

Contact us

Do you have questions about our products or services? We’re here to help. Contact a Roche representative in your region.

Contact us

KAPA products are for research use only, not for use in diagnostics procedures.

The AVENIO Edge System is a Class 1 US IVD and a Class A CE IVD (under IVDR) and is intended for downstream diagnostic applications. For the EU, Roche is collaborating with Platomics to enable laboratories to help automate document generation on Platomics’ multi-stakeholder platform for workflows incorporating the Avenio Edge System. Reagents and kits mentioned are for Research Use Only. They are not intended for diagnostic procedures. The AVENIO Edge System can be used for Research Use Only (RUO) workflows.