NGS target enrichment solutions

NGS target enrichment focuses sequencing resources on specific genomic regions using methods like hybrid capture or amplicon-based approaches. By eliminating regions not relevant to a particular study, this technique offers powerful advantages over whole-genome sequencing by dramatically reducing costs and the computational burden of analysis.¹

This focused approach enables far greater sequencing depth of selected regions, which increases statistical power and prevents false data interpretations—especially in samples with limited input. The resulting sensitivity is invaluable for detecting low-frequency somatic mutations in heterogeneous samples, identifying relevant variants, and enabling cost-effective gene expression studies in oncology research applications. Its power and flexibility have established NGS target enrichment as a cornerstone of modern genomics research and translational medicine.

At Roche, we empower researchers with comprehensive and innovative target enrichment solutions for NGS, from extensive catalog panels to fully customized designs. We help you overcome the toughest sequencing challenges, delivering precise and sensitive results from low-input samples and difficult-to-capture genomic targets. Our goal is to accelerate your success, enabling a deeper understanding of underlying mechanisms, improving clinical research capabilities, and unlocking the potential of new applications.

Roche's NGS target enrichment solutions provide exceptionally competitive and superior coverage across key genomic databases. Our meticulously designed solutions ensure robust interrogation of critical genomic regions, minimizing dropouts and maximizing the depth of sequencing data. This comprehensive coverage translates directly to increased confidence in variant calling results, enabling more reliable downstream analysis and accelerating research insights. Highlighted here:

• KAPA HyperExome V2 Probes deliver superior coverage of the recent versions of key genomic databases

Roche offers both hybridization-based and primer extension NGS target enrichment solutions that are part of a fully integrated and validated workflow. Our solutions incorporate high-quality, expertly designed probes or primers, as well as the streamlined KAPA library prep kits, ensuring all components work together seamlessly to maximize performance and minimize technical variability.

This comprehensive approach enables the focusing of sequencing resources for targeted resequencing applications, delivering the high uniformity and sensitivity critical for research in human genetic disease and oncology. The workflows include:

• KAPA HyperCap Workflow

• KAPA HyperPETE Workflow