{ "ProductData": { "ID": "RTD000913", "ProductType": "Others", "BrandName": "FITC anti-IgG Primary Antibody", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use.", "DisclaimerGroup1": "IVD", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "Frozen tissue" ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "Frozen, Tonsil or Lymph Node Clotted Blood" ], "ISOtypes": "", "Species": [ "Goat" ], "StainLocalization": [ "" ], "ProductNameGlobal": "IgG Primary Antibody FITC" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Product_RTD000913_im_en.jpg", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "05-0002", "StructureNodeStatus": "Active", "NodeName": "IHC Primary Antibodies" }, { "StructureSystemIdentifier": 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Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "This antibody is intended for in vitro diagnostic (IVD) use.

Ventana® Medical Systems’ (Ventana) Systems FITC anti-IgG (immunoglobulin G) Primary Antibody is a goat derived polyclonal antibody labeled with fluorescein and specifically directed against human IgG. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of immunoglobulin G in IgG target tissue (e.g., in the diagnosis of renal or dermal pathologies). FITC anti-IgG is intended for laboratory use to qualitatively stain sections of frozen tissue on a Ventana automated slide stainer.

The clinical interpretation of any staining, or the absence of staining, must be complemented by morphological studies and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests. Prescription only.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Content", "Value": "FITC anti-IgG contains sufficient reagent for 50 tests.

One 5 mL dispenser of FITC anti-IgG; contains approximately 817 µg (163.4 µg/mL) of a goat polyclonal antibody directed against human IgG. The antibody is diluted in a tris based buffer containing carrier protein and preservative.
Total protein concentration of the reagent is approximately 600 µg/mL.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Background Information", "Value": "Fluorescent antibodies have been used to detect specific antigens in cells or tissue for over 40 years.1 An informative overview of the use of FITC conjugated antibodies as effective and specific immunofluorescent markers for cellular antigens may be found in Faulk and Humans.2 Use of the immunofluorescence technique has resulted in an increased overall understanding of renal,3 and dermal4 pathologies.

FITC anti-IgG contains a goat polyclonal antibody raised against purified human IgG. The antibody is obtained through purification of the goat gamma globulin fraction, followed by reaction with fluorescein isothiocyanate. The excess fluorochrome is then removed by dialysis and the conjugated globulin is further fractionated on DEAE cellulose to remove over and under labeled protein.5 Immunofluorescent detection of IgG in human renal6,7,8 and dermal9,4 tissue has been described. Anti-IgG binds specifically with the heavy chain portions of human immunoglobulin G.

1. Coons AH, Leduc EH, Kaplan MH. Localization of antigen in tissue cells. VI. The fate of injected foreign proteins in the mouse. J Exp Med. Feb;93(2):173-88, 1951.
2. Faulk WP, Hijmans W. Recent developments in immunofluorescence. Prog Allergy.;16:9-39, 1972.
3. McCluskey RT, Collins AB. The value of immunofluorescence in the study of renal disease. Ann N Y Acad Sci.;420:302-8, 1983.
4. Wick MR, Ritter JH, Humphrey PA, Swanson PE. Immunopathology of nonneoplastic skin disease: a brief review. Am J Clin Pathol. Apr;105(4):417-29, 1996.
5. Wood BT, Thompson SH, Goldstein G. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies. J Immunol. Aug;95(2):225-9, 1965.
6. Tomino Y, Sakai H, Miura M, Endoh M, Nomoto Y. Detection of polymeric IgA in glomeruli from patients with IgA nephropathy. Clin Exp Immunol. Aug;49(2):419-25, 1982.
7. Inoue W, Tomino Y, Miura M, Yagame M, Nomoto Y, Sakai H. Detection of immunoglobulins and other serum proteins in the dermal and glomerular capillary walls from patients with diabetes mellitus. Acta Pathol Jpn. Aug;36(8):1181-9, 1986.
8. Tokuda M, Shimizu J, Sugiyama N, Kiryu T, Matsuoka K, Sasaki O, Fukuda K, Hatase O, Monden H. Direct evidence of the production of IgA by tonsillar lymphocytes and the binding of IgA to the glomerular mesangium of IgA nephropathy patients. Acta Otolaryngol Suppl.523:182-4, 1996.
9. Chan LS, Traczyk T, Taylor TB, Eramo LR, Woodley DT, Zone JJ. Linear IgA bullous dermatosis. Characterization of a subset of patients with concurrent IgA and IgG anti-basement membrane autoantibodies. Arch Dermatol. 1995 Dec;131(12):1432-7.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "FITC anti-IgG may be used as the primary antibody for immunohistochemical staining of frozen tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. For FITC direct labeled antibodies the fluorochrome is linked to the primary antibody and therefore no secondary antibody or chromogenic detection step is required. The primary antibody binds specifically to the target antigen and can then be visualized. Results are interpreted using a fluorescent microscope with the appropriate filter set and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

FITC anti-IgG is optimally diluted for use with automated slide stainers. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the Ventana automated slide stainer to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate Ventana automated slide stainer operator's manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "This antibody is intended for in vitro diagnostic (IVD) use.

Ventana® Medical Systems’ (Ventana) Systems FITC anti-IgG (immunoglobulin G) Primary Antibody is a goat derived polyclonal antibody labeled with fluorescein and specifically directed against human IgG. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of immunoglobulin G in IgG target tissue (e.g., in the diagnosis of renal or dermal pathologies). FITC anti-IgG is intended for laboratory use to qualitatively stain sections of frozen tissue on a Ventana automated slide stainer.

The clinical interpretation of any staining, or the absence of staining, must be complemented by morphological studies and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests. Prescription only.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }

FITC anti-IgG Primary Antibody

IVD For in vitro diagnostic use.
FITC anti-IgG Primary Antibody

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