For in vitro diagnostic use. Others ISH iVIEWBlue Plus Detection Kit IVD ISH iVIEWBlue Plus Detection Kit RTD000978 Ventana Medical Systems, Inc.’s (Ventana) ISH iVIEW Blue Plus Detection Kit consists of a primary rabbit anti-DNP antibody followed by an indirect biotin streptavidin system for detecting VENTANA DNP labeled probes and antibodies. This kit is intended for staining sections of routinely fixed (neutral buffered formalin), paraffin embedded tissue on the BenchMark series automated slide stainers.Light microscopy is used to detect the staining intensity. Positive results aid in the classification of normal and abnormal cells and tissues and serve as an adjunct to conventional histopathology tests.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in vitro diagnostic (IVD) use. en ISH iVIEW Blue Plus Detection Kit detects specific DNP labeled probes and antibodies bound to a target sequence or antigen in paraffin-embedded tissue sections. The labeled probe or antibody is located by an anti-DNP antibody, then by an enzyme-labeled secondary antibody or a biotin-conjugated secondary antibody. This step is followed by the addition of a Streptavidin-AP (alkaline phosphatase) enzyme conjugate which binds to the biotin present on the secondary antibody. The complex is then visualized with 5- Bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) chromogen, which produces a blue precipitate that is readily detected by light microscopy.The staining protocol consists of numerous steps in which reagents are incubated for predetermined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip, which minimizes the evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with positive staining for the probe or antibody. en

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ISH iVIEW_Blue Plus Detection Kit

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