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Light microscopy is used to detect the staining intensity. Positive results aid in the classification of normal and abnormal cells and tissues and serve as an adjunct to conventional histopathology tests.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
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One 10mL dispenser ISH
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Blue Plus Anti-DNP contains rabbit anti-DNP antibody in phosphate buffered saline with protein stabilizer and ProClin 300, a preservative(<1%).
One 10 mL dispenser ISH
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Blue Plus Streptavidin Alkaline Phosphatase contains enzyme conjugate inTris buffer with MgCl
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One 10mL dispenser ISH
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Blue Plus NBT contains nitro blue tetrazolium in <1 % dimethylformamide (DMF).
One 10 mL dispenser ISH
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Blue Plus BCIP contains 5-bromo-4-chloro-3- indolyl phosphate in aTris buffer(<1%).
One 10 mL dispenser ISH
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Blue Plus Enhancer containsan MgCl
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Blue Plus Amp contains mouse anti-rabbit antibody in phosphate buffered saline with protein stabilizer and ProClin 300, a preservative (<1%).
One 10 mL dispenser ISH
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Blue Plus Biotinylated Ig contains affinity purified goat anti-mouse IgG in phosphate buffered saline with protein stabilizer and ProClin 300, a preservative (<1%).
One 10 mL dispenser ISH
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Blue Plus HybReady contains prehybridization buffer with formamide (<57% CH
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in situ hybridization (ISH) uses labeled probes to detect specific DNA or RNA target sequences in fixedor frozen tissue sections. Target sequences are exposed by heating the tissue and probe solution to denature nucleic acids. The reaction is then cooled allowing the labeled nucleic acid probe to hybridize to its complementary nucleic acid sequence in the tissue.
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Immunohistochemistry (IHC) is a technique used in laboratories for diagnostic purposes. IHC uses specific primary antibodies to localize and bind to antigens in fixed or frozen tissue sections or cytology specimens. The binding of the antibody to the antigen is visualized with an indirect detection method. The most common techniques for indirect methods use a secondary antibody directed against the species of primary antibody and an enzyme with a corresponding substrate-chromogen system. This combination results in a colored precipitate at the site of specific antibody binding. ISH
iVIEW
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The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark Series automated slide stainer (BenchMark, BenchMark GX, and BenchMark XT)washes the sections to remove unbound material and applies a liquid coverslip, which minimizes the evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with positive staining for the probeor antibody.
For more detailed information on instrument operation, refer to the appropriate Operator's Manual.
Figure 1 illustrates the indirect detection method.
Figure 1. VENTANA ISH
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{
"Name": "Product Purpose",
"Value": "Ventana Medical Systems, Inc.’s (Ventana) ISH
iVIEW
Blue Plus Detection Kit consists of a primary rabbit anti-DNP antibody followed by an indirect biotin streptavidin system for detecting VENTANA DNP labeled probes and antibodies. This kit is intended for staining sections of routinely fixed (neutral buffered formalin), paraffin embedded tissue on the BenchMark Series automated slide stainers.
Light microscopy is used to detect the staining intensity. Positive results aid in the classification of normal and abnormal cells and tissues and serve as an adjunct to conventional histopathology tests.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
This reagent is intended for
in vitro diagnostic (IVD) use.",
"Language": "en",
"Country": "XG",
"Code": "Product Purpose"
}
]
}
}
]
}
